利用新型荧光银纳米团簇实现谷胱甘肽的快速精确检测

发布时间：2018-03-25 21:21

本文选题：荧光光谱分析　切入点：谷胱甘肽检测　出处：《光谱学与光谱分析》2016年12期

【摘要】：谷胱甘肽(GSH)是一种含有巯基的三肽分子,参与许多细胞内生化过程,具有抗氧化和整合解毒功能,在生物体内以及医学,食品等领域有着极为重要的作用。GSH参与细胞内、体液中的许多重要生化反应,其在人体内含量的变化,相应地提示了人体的健康问题。目前对GSH的检测手段有表面增强拉曼光谱(SERS)、电化学分析、高效液相色谱(HPLC)等,这些方法大都操作复杂、耗时较长或者需要昂贵的仪器。利用一种新型荧光银纳米团簇(Ag NCs)作为探针,通过同时分析银纳米团簇的荧光强度变化以及荧光峰位置移动实现了GSH的高精度快速检测。在检测过程中,GSH分子与荧光探针发生化学反应,改变了荧光探针的光化学特性,其荧光强度因发生猝灭而减弱,且其荧光峰位置因配体的改变也发生移动。通过对照组实验,我们进一步证明了所发展的检测方法对GSH目标具有很好的特异性,综合考察荧光强度和波长的变化数据可以很好地区分GSH以及其他结构类似的分子,同时探针对于多种盐离子及氨基酸等不敏感,能够很好地保证检测的准确性。我们报导的荧光探针合成步骤简单,过程绿色环保,GSH检测的响应速度快、光谱波动较小、相对误差小。进一步的研究有望实现细胞内的GSH高精度检测及成像。
[Abstract]:Glutathione glutathione (GSH) is a tripeptide molecule containing sulfhydryl group, which is involved in many intracellular biochemical processes and has anti-oxidation and detoxification functions. GSH plays an extremely important role in biology, medicine, food and other fields. Many important biochemical reactions in body fluid, the changes of their contents in the human body indicate the health problems of human body accordingly. At present, the detection methods of GSH include surface-enhanced Raman spectroscopy, electrochemical analysis, high performance liquid chromatography (HPLC), etc. Most of these methods are complex, time-consuming or expensive instruments, using a new fluorescent silver nanocluster, Ag NCs, as a probe. The high precision and fast detection of GSH was realized by simultaneously analyzing the fluorescence intensity change of silver nanoclusters and the shift of fluorescence peak position. The photochemical properties of the fluorescent probe were changed by the chemical reaction between the GSH molecule and the fluorescence probe. The fluorescence intensity was weakened by quenching, and the position of fluorescence peak was shifted because of the change of ligands. Through the experiment of control group, we further proved that the developed detection method has good specificity for GSH target. The fluorescence intensity and wavelength variation data can be used to distinguish GSH and other molecules with similar structure, and the probe is insensitive to many salt ions and amino acids. It can ensure the accuracy of the detection. The synthetic steps of fluorescence probe reported by us are simple, the response speed of GSH detection of green environmental protection is fast, and the spectral fluctuation is relatively small. The relative error is small. Further research is expected to achieve high-precision detection and imaging of GSH in cells.
【作者单位】：精密光谱科学与技术国家重点实验室华东师范大学;
【基金】：国家自然科学基金项目(61178085) 上海市自然科学基金项目(15ZR1410100)资助
【分类号】：O657.3;R927

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