二维高效液相色谱法测定血浆中氨甲环酸血药浓度方法的建立及其在关节置换术患者中的应用

发布时间：2018-03-25 21:24

  本文选题：氨甲环酸　切入点：二维高效液相色谱　出处：《中国医院药学杂志》2016年23期

【摘要】：目的:建立全自动二维色谱法(2D-LC-UV)测定血浆中氨甲环酸浓度的方法,用于临床上行关节置换术患者围术期氨甲环酸治疗药物浓度的监测。方法:待测物在一维柱ASTON SPX(100 mm×4.6 mm,5μm)上初步分离,通过中间柱ASTO N SHC(10 mm×4.6 mm,3μm)截取保留并在二维柱ASTON SX1(150 mm×4.6 mm,5μm)上进一步分离,200μL样品进样,并最终测定。流速均为1.0mL·min~(-1),柱温为40℃,紫外检测波长为220 nm。所建立方法运用于58名行膝关节/髋关节置换术的患者围术期中氨甲环酸血浆样本的检测分析。结果:在所建立的色谱条件下,氨甲环酸与各杂质分离良好,线性范围为5~300μg·mL~(-1),最低检测限为5μg·mL~(-1),批间批间精密度均小于6.6%,提取回收率大于72.4%。58名患者围术期中氨甲环酸首次给药后3个时间点(5 min,30 min和2 h)平均血浆浓度分别为:(103.3±20.4)μg·mL~(-1),(53.0±14.8)μg·mL~(-1)和(22.8±8.3)μg·mL~(-1)。手术中男女之间氨甲环酸血药浓度无显著性差异(P0.05),而不同手术种类之间(膝关节/髋关节置换术)氨甲环酸血药浓度存在显著性差异(P0.05)。结论:此法操作简单,准确、精密度好,适用于临床氨甲环酸血浆浓度的监测及浓度-疗效关系研究。
[Abstract]:Objective: to establish a method for the determination of carbamate in plasma by automatic two-dimensional chromatography (2D-LC-UV). To monitor the perioperative concentration of methionine in clinical patients undergoing arthroplasty. Methods: the substance to be tested was preliminarily separated on one dimensional column ASTON SPX(100 mm 脳 4. 6 mm ~ 5 渭 m. ASTO N SHC(10 mm 脳 4. 6 mm ~ (3 渭 m) was used to intercept and retain the sample and to separate the sample on a two dimensional column (ASTON SX1(150 mm 脳 4. 6 mm ~ 5 渭 m). The results were as follows: the flow rate was 1.0mL min-1n, the column temperature was 40 鈩，

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