阿加曲班对脑出血模型大鼠脑损伤的改善作用

发布时间：2018-03-29 12:11

本文选题：阿加曲班　切入点：脑出血　出处：《中国药房》2015年19期

【摘要】：目的:研究阿加曲班对脑出血模型大鼠脑损伤的改善作用。方法:取50只SD大鼠随机均分为正常对照(等量生理盐水)组、模型(等量生理盐水)组和阿加曲班低、中、高剂量(0.75、1.5、3 mg/kg)组,除正常对照组外其余各组大鼠复制脑出血模型。复制模型30 min后各组大鼠ip给予相应药物,每天1次,连续给药3 d。处死大鼠,检测各组大鼠脑组织中肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)含量,半胱氨酸天冬氨酸蛋白水解酶3(Caspase-3)活性,Bcl-2、Bax、基质金属蛋白酶2(MMP-2)、MMP-9蛋白表达和核转录因子κB(NF-κB)p65、IκBα的磷酸化水平。结果:与正常对照组比较,模型组大鼠脑组织中TNF-α、IL-1β含量增加,Caspase-3活性和MMP-2、MMP-9、Bax蛋白表达增强,Bcl-2蛋白表达减弱,NF-κB p65、IκBα磷酸化水平升高,差异均具有统计学意义(P0.01)。与模型组比较,阿加曲班低、中、高剂量组大鼠脑组织中TNF-α、IL-1β含量减少,Caspase-3活性和MMP-2、MMP-9、Bax蛋白表达减弱,Bcl-2蛋白表达增强,NF-κB p65、IκBα磷酸化水平降低,差异均具有统计学意义(P0.01或P0.05),且与剂量呈正相关。结论:阿加曲班呈剂量依赖性地抑制脑出血模型大鼠炎症因子分泌及脑组织细胞凋亡,其作用可能与NF-κB信号通路有关。
[Abstract]:Objective: to study the effect of Agatripine on cerebral injury in rats with intracerebral hemorrhage. Methods: fifty SD rats were randomly divided into normal control group, model group and Agatripine group. The rats in the high dose group (0.75 mg / kg) were given intraperitoneal administration of the corresponding drugs once a day for 3 days. The rats were killed for 3 days, except the normal control group, and the rats in each group were given IP for 30 min after the establishment of the model. The contents of TNF- 伪 TNF- 伪 and IL-1 尾 in brain tissue of rats in each group were determined. Activity of cysteine aspartate protein hydrolase 3 (caspase-3) and expression of MMP-9 and phosphorylation of nuclear transcription factor 魏 B, NF- 魏 B, p65, I 魏 B 伪 in matrix metalloproteinase 2, matrix metalloproteinase 2, MMP-2, and MMP 2 were compared with those in normal control group. In the model group, the content of TNF- 伪 and IL-1 尾 increased the activity of Caspase-3 and the expression of MMP-2, MMP-9 and Bax protein increased. The expression of Bcl-2 protein decreased and the level of phosphorylation of NF- 魏 B p65, I 魏 B 伪 increased, and the difference was statistically significant (P 0.01). Compared with the model group, Agatreban was low and moderate. In high dose group, the contents of TNF- 伪 and IL-1 尾 decreased the activity of caspase-3 and the expression of MMP-2, MMP-9 and Bax protein decreased, the expression of Bcl-2 protein increased, and the level of phosphorylation of NF- 魏 B p65 I 魏 B 伪 decreased. The difference was statistically significant (P0.01 or P0.05A), and was positively correlated with the dose. Conclusion: Agatroban can inhibit the secretion of inflammatory factors and apoptosis of brain tissue in a dose-dependent manner in rats with intracerebral hemorrhage, and its effect may be related to NF- 魏 B signaling pathway.
【作者单位】：天津市武清区人民医院神经内科;天津市武清区人民医院消化内科;
【分类号】：R965;R-332

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