活性维生素D3对进展性肾炎大鼠肾小管间质损伤的干预作用

发布时间：2018-03-30 02:40

本文选题：活性维生素D　切入点：低氧诱导因子-　出处：《中国老年学杂志》2015年06期

【摘要】：目的探讨活性维生素D3对进展性肾炎大鼠肾小管间质损伤的干预作用及对肾间质纤维化的影响。方法实验动物按数字随机表法分为假手术组、进展性肾炎组(模型组)、活性维生素D3干预组(治疗组),每组7只。除对照组外,均采用右肾切除并两次注射Ig G(OX-7)鼠抗人Thy1.1单克隆抗体制备进展性肾炎大鼠模型。活性维生素D3干预8 w后,检测各组大鼠24 h尿蛋白、血肌酐(Scr)、尿素氮(BUN)及电解质的变化,并观察肾组织病理改变。免疫荧光检测肾小管间质低氧诱导因子(HIF)-1α的表达,原位杂交检测肾小管间质结缔组织生长因子(CTGF)mRNA的表达。结果与模型组比较,活性维生素D3治疗不仅使HIF-1α、CTGF表达显著下调(P0.05),还能使大鼠24 h尿蛋白、BUN明显降低(P0.05),肾组织病理损害明显减轻。结论慢性低氧上调了HIF-1α及前纤维因子的表达,活性维生素D3可能通过明显降低进展性肾炎大鼠肾小管间质HIF-1α、CTGF的表达,减轻肾小管间质纤维化而发挥肾脏保护作用。
[Abstract]:Objective to investigate the effect of active vitamin D 3 on renal tubulointerstitial injury and renal interstitial fibrosis in rats with progressive nephritis.Methods Experimental animals were randomly divided into sham-operated group, progressive nephritis group (model group) and active vitamin D3 intervention group (7 rats in each group).Except the control group, the rat model of progressive nephritis was established by right nephrectomy and twice injection of Ig GnOX-7) mouse monoclonal antibody against human Thy1.1.After intervention with active vitamin D _ 3 for 8 weeks, the changes of urine protein, creatinine creatinine, bun (bun) and electrolytes in 24 h were detected, and the pathological changes of renal tissue were observed.The expression of tubulointerstitial hypoxia inducible factor (HIF-1 伪) and tubulointerstitial connective tissue growth factor (CTGFN) mRNA were detected by immunofluorescence and in situ hybridization respectively.Results compared with the model group, the active vitamin D _ 3 treatment not only significantly decreased the expression of HIF-1 伪 -CTGF, but also significantly decreased the urine protein bun at 24 h and the pathological damage of renal tissue.Conclusion chronic hypoxia up-regulates the expression of HIF-1 伪 and prefibroin, and active vitamin D 3 may play a role in renal protection by significantly reducing the expression of HIF-1 伪 and CTGF in renal tubulointerstitial of rats with progressive glomerulonephritis.
【作者单位】：贵州省人民医院肾内科;毕节市第一人民医院肾内科;贵州省人民医院病理科;
【基金】：国家自然科学基金(No.81200539) 国际科技合作计划项目(黔科合外G字〔2012〕7045号) 2012年省长基金(No.黔省专合字(2012)128号) 2010年省长基金(No.黔省专合字(2010)89号)
【分类号】：R965

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