胰岛素自微乳剂肠道给药的降血糖效果考察

发布时间：2018-03-31 03:42

本文选题：胰岛素　切入点：自微乳剂　出处：《中国药房》2015年19期

【摘要】：目的:研究胰岛素自微乳剂肠道给药在1型糖尿病模型大鼠体内的降血糖效果。方法:取大鼠ip给予链脲佐菌素(50mg/kg)复制1型糖尿病模型,将模型大鼠随机分为模型(生理盐水)组、阳性对照(胰岛素注射液2.25 u/kg)组和微乳剂低、中、高剂量组(胰岛素自微乳剂4.5、9、18 u/kg);另取正常大鼠10只为假手术(生理盐水)组。均麻醉手术,除阳性对照组大鼠ip给药外,其余大鼠肠道给药,于给药前和给药后15~600 min检测各组大鼠血糖水平。另分别对正常对照组、模型未给糖组、模型给糖组和微乳剂(胰岛素自微乳剂9 u/kg)组大鼠进行糖耐量实验(除模型未给糖组大鼠不给糖外其余大鼠均给糖)。结果:与假手术组比较,模型组大鼠给药后0~240 min内的血糖值均增加,差异具有统计学意义(P0.05)。与模型组比较,阳性对照组大鼠给药15 min后有降血糖趋势,给药后30~480 min内的血糖值均减小,其中降血糖峰值为36%、峰值时间为30 min;微乳剂低、中、高剂量组大鼠给药30 min后有降血糖趋势,给药后45~360 min内的血糖值均减小,其中降血糖峰值为18%~21%、峰值时间为90~120min,以上差异具有统计学意义(P0.01或P0.05)。糖耐量实验中除模型未给糖组外其余各组大鼠均有血糖吸收峰,且微乳剂组大鼠血糖达峰值后迅速降低。结论:胰岛素自微乳剂能明显降低1型糖尿病模型大鼠的血糖。
[Abstract]:Objective: to study the hypoglycemic effect of insulin self-microemulsion in rats with type 1 diabetes mellitus. Methods: the model of type 1 diabetes was induced by intravenous administration of streptozotocin (50 mg / kg). The model rats were randomly divided into two groups: the model group (normal saline), the positive control group (2.25 u / kg insulin injection) and the low and moderate microemulsion group. The rats in the high dose group (4.5 渭 g / kg insulin self-microemulsion) and 10 normal rats were sham-operated (normal saline) group. All the rats were anesthetized, and the other rats were given intestinal administration except IP in the positive control group. The blood glucose levels of rats in each group were measured before and 15 min after administration. The glucose tolerance test was performed in the model group and the self-emulsion group (9 u / kg insulin self-emulsion). (all rats except the model group were given no glucose. Results: compared with the sham operation group, the rats in the model group were given no glucose. The blood glucose levels in the model group increased within 0 ~ 240 min after administration, and the difference was statistically significant (P 0.05). Compared with the model group, there was a tendency of hypoglycemia in the positive control group after 15 min administration, and a decrease in the blood glucose value within 30 ~ 480 min after the administration of the drug. The peak value of hypoglycemia was 36 min, and the peak time was 30 mins. In the low, medium and high dose groups, there was a tendency of hypoglycemia after 30 min administration, and the blood glucose values decreased within 45 ~ 360 min after administration. The peak value of hypoglycemia was 18 ~ 21 and the peak time was 90 ~ 120 min. The difference was statistically significant (P 0.01 or P 0.05). In the glucose tolerance experiment, there was a peak of blood glucose absorption in all the rats except the model group. Conclusion: insulin self-microemulsion can significantly decrease blood glucose in rats with type 1 diabetes mellitus.
【作者单位】：广东省人民医院/广东省医学科学院;广东医学实验动物中心;
【基金】：广东省科技计划基金(No.2011B031800010)
【分类号】：R965

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