氮乙酰半胱氨酸对压力负荷高血压大鼠心肌肥厚的影响及其抗炎机制

发布时间：2018-04-02 05:10

本文选题：心肌肥厚　切入点：炎性细胞因子　出处：《中国临床药理学杂志》2015年16期

【摘要】：目的观察氮乙酰半胱氨酸(NAC)对心肌肥厚的作用并探讨其与炎性因子表达的关系。方法 SD大鼠随机分为4组,假手术组、模型组、假手术药物组及模型药物组,腹主动脉缩窄法建立压力负荷模型。药物组灌胃NAC(0.2 g·kg-1·d-1)干预8周。通过组织病理和心脏超声评估心肌的肥厚程度,比色法检测胶原含量,RT-PCR和ELISA法测定炎性细胞因子表达水平,用Fenton原理测定心肌内活性氧(ROS)水平。结果与假手术组比较,模型组大鼠的血压升高,且左室重量指数、心肌细胞内径、胶原含量及左室室壁厚度均明显升高(P0.05);心肌内的肿瘤坏死因子(TNF-α)、白细胞介素-6(IL-6)mRNA和蛋白水平也显著升高(P0.05)。NAC干预后,心肌组织的ROS水平明显下降,左室重量指数、胶原含量以及左室室壁厚度均较模型组明显降低(P0.05)。模型药物组的TNF-α、IL-6 mRNA和蛋白水平均较模型组显著下降(P0.05);但IL-10蛋白含量与假手术组比较差异不显著。结论 NAC可改善压力负荷大鼠心肌肥厚、抑制促炎性细胞因子表达可能是其重要分子机制之一。
[Abstract]:Objective to investigate the effect of NAC- NACon on myocardial hypertrophy and its relationship with the expression of inflammatory factors.Methods SD rats were randomly divided into 4 groups: sham operation group, model group, sham-operation drug group and model drug group.The drug group was treated with NAC(0.2 g kg-1 d -1 for 8 weeks.The degree of myocardial hypertrophy was assessed by histopathology and echocardiography, collagen content was measured by colorimetric assay, inflammatory cytokines were detected by RT-PCR and ELISA, and reactive oxygen species (Ros) levels in myocardium were measured by Fenton principle.Results compared with the sham-operated group, the blood pressure of the model group was increased, and the left ventricular mass index (LVMI) and the inner diameter of cardiac myocytes were also increased in the model group.The content of collagen and the thickness of left ventricular wall increased significantly (P 0.05), and the levels of TNF- 伪 and IL-6 in myocardium also increased significantly after intervention with P0.05. NAC, the ROS level of myocardial tissue decreased and left ventricular mass index decreased.Collagen content and left ventricular wall thickness were significantly decreased compared with the model group (P 0.05).The levels of TNF- 伪 IL-6 mRNA and protein in the model group were significantly lower than those in the model group, but the content of IL-10 protein was not significantly different from that in the sham operation group.Conclusion NAC can improve myocardial hypertrophy and inhibit the expression of pro-inflammatory cytokines, which may be one of its important molecular mechanisms.
【作者单位】：第四军医大学唐都医院心脏内科;第四军医大学唐都医院药剂科;
【基金】：陕西省社会发展攻关课题基金资助项目(2012K15-01-06)
【分类号】：R965

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