瑞芬太尼聚己内酯对脊髓缺血再灌注损伤中运动诱发电位的影响

发布时间：2018-04-03 18:12

本文选题：脊髓缺血再灌注损伤　切入点：Mu阿片受体　出处：《中国医院药学杂志》2015年12期

【摘要】：目的:应用诱发电位监测脊髓电生理的改变,探讨腹主动脉内灌注Mu阿片受体激动剂REM-PCL对Mu阿片受体信号转导介导的神经元保护效果。通过建立兔脊髓缺血再灌注损伤(SCIRI)模型,腹主动脉内灌注Mu阿片受体激动剂REMPCL和选择性Mu阿片受体拮抗剂GSK1521498对Mu阿片受体信号转导介导的神经元保护效果和作用机制进行探讨,并应用诱发电位监测脊髓电生理的改变以及REM-PCL干预后的影响,为临床脊髓保护提供理论和实验依据。方法:30只健康新西兰大白兔随机分为对照组(C组)、REM-PCL组(RP组,0.1 mg·kg-1)和REM-PCL+GSK1521498组(RG组,0.1 mg·kg-1REM-PCL+1 mg·kg-1GSK1521498),每组10只。采用肾下腹主动脉阻断法,建立兔脊髓缺血再灌注损伤(SCIRI)模型。分别于阻断前、再灌注15 min、再灌注30 min、再灌注60 min及再灌注24 h监测血清神经特异性烯醇化酶浓度和MEP变化。再灌注24 h神经行为学评定后取脊髓组织检测Mu阿片受体mRNA表达情况并观察其病理学改变。结果:运动诱发电位可准确反映SCIRI程度。C和RG组血清NSE浓度明显增加而Mu阿片受体mRNA表达明显降低(P0.01),MEP和脊髓灰质病理损害严重(P0.01)。RP组血清NSE浓度明显降低而Mu阿片受体mRNA表达明显增加(P0.01),MEP和脊髓灰质的病理损害程度明显轻于C和RG组(P0.01),神经行为学评分恢复迅速(P0.01)。结论:SCIRI能导致脊髓电生理功能损害,应用REM-PCL可以通过激活Mu阿片受体减轻脊髓缺血再灌注损伤。
[Abstract]:Objective: the application of evoked potential monitoring electrophysiological changes of spinal cord, abdominal aorta perfusion of Mu opioid receptor agonist REM-PCL on neuron protective effect of Mu opioid receptor mediated signal transduction. Through the establishment of rabbit spinal cord ischemia reperfusion injury (SCIRI) model, abdominal aorta perfusion Mu opioid receptor agonist REMPCL and the selective Mu opioid receptor antagonist GSK1521498 on Mu opioid receptor signal transduction mediated neuroprotective effect and mechanism are discussed, and the application of evoked potential monitoring electrophysiological changes of spinal cord and REM-PCL intervention effect, provide theoretical and experimental basis for clinical spinal cord protection. Methods: 30 healthy New Zealand rabbits were randomly divided into the control group (C group), REM-PCL group (group RP, 0.1 mg kg-1) and REM-PCL+GSK1521498 group (group RG, 0.1 mg - kg-1REM-PCL+1 Mg - kg-1GSK1521498), 10 rats in each group. Using the infrarenal aortic resistance Breaking method, establishment of spinal cord ischemia reperfusion injury in rabbits (SCIRI) model. Respectively before occlusion, reperfusion 15 min, reperfusion 30 min, reperfusion 60 min and reperfusion 24 h monitoring of serum neuron specific enolase concentration changes of MEP and 24 h of reperfusion Neurobehavioral Evaluation after the detection of Mu spinal cord opioid receptor mRNA expression and observe the pathological changes. Results: motor evoked potentials can accurately reflect the degree of SCIRI and.C in RG group serum NSE concentration significantly increased and the expression of Mu opioid receptor mRNA decreased significantly (P0.01), MEP and pathological damage of spinal cord (P0.01) in.RP group significantly decreased the concentration of serum NSE the expression of Mu opioid receptor mRNA increased significantly (P0.01), the degree of pathological damage of MEP and spinal cord was less than C and RG group (P0.01), the neurobehavioral scores rapid recovery (P0.01). Conclusion: SCIRI can lead to spinal cord function damage, REM-PCL can be used Through the activation of Mu opioid receptor in spinal cord ischemia reperfusion injury.

【作者单位】：贵阳医学院附属人民医院麻醉科;四川大学华西医院麻醉科;
【基金】：贵州省卫生厅基金资助项目(编号:gzwkj2010-1-006,gzwkj2012-1-051) 贵州省科技厅基金资助项目[编号:黔科合SY字(2011)008号,黔科SY字(2012)3090号]
【分类号】：R965

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