液相色谱-串联质谱法测定人血浆中奥美拉唑的血药浓度及其药动学

发布时间：2018-04-03 18:21

  本文选题：奥美拉唑　切入点：液相色谱-串联质谱法　出处：《中国医院药学杂志》2015年14期

【摘要】：目的:建立液相色谱-串联质谱法测定人血浆中奥美拉唑的血药浓度并进行药动学研究。方法:以兰索拉唑为内标,血浆样品经乙腈沉淀后,经LC-MS/MS分离分析。采用Diamonsil C18柱(2.1 mm×150 mm,5μm),甲醇-水(含0.01%甲酸)(67∶33)为流动相;流速为0.3 ml·min-1,采用电喷雾离子源(ESI),以多反应监测方式(MRM)进行负离子监测,奥美拉唑和兰索拉唑的定量分析离子对分别为m/z 344.2/193.8,367.9/163.8。结果:奥美拉唑在3.38~2 110.00ng·ml-1(r=0.997 1)范围内线性关系良好,最低定量限为3.38 ng·ml-1,方法回收率在92.48%~99.39%,日内(n=5)RE均小于3.96%,日间(n=15)RE均小于6.41%。结论:该方法快速简便,灵敏准确,可用于奥美拉唑血药浓度监测和药动学研究。
[Abstract]:Objective: to establish a liquid chromatography-tandem mass spectrometry (LC-MS) method for the determination of omeprazole in human plasma and its pharmacokinetics.Methods: Lansoprazole was used as internal standard. Plasma samples were precipitated by acetonitrile and separated by LC-MS/MS.A Diamonsil C18 column of 2.1 mm 脳 150mm ~ 5 渭 m ~ (-1) with methanol-water (containing 0.01% formic acid 67: 33) as mobile phase, a flow rate of 0.3 ml / min ~ (-1) and an electrospray ion source (ESI) was used to monitor negative ions.The quantitative ion pairs of omeprazole and lansoprazole were m / z 344.2 / 193.8367.9 / 163.8, respectively.缁撴灉:濂ョ編鎷夊攽鍦，

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