米诺环素对大鼠脑缺血再灌注损伤后学习记忆能力及海马神经元PSD-95蛋白表达的影响

发布时间：2018-04-04 19:24

  本文选题：米诺环素　切入点：学习记忆　出处：《重庆医科大学学报》2015年01期

【摘要】：目的:探讨静脉用低剂量米诺环素对局灶性脑缺血再灌注大鼠认知功能障碍及缺血侧海马生长相关蛋白-43(growth associated protein-43,GAP-43)和突触后致密物质-95(postsynaptic density 95,PSD-95)表达的影响。方法:采用线栓法建立Sprague-Dawley大鼠大脑中动脉阻塞再灌注模型,将45只雄性大鼠随机分为假手术组、模型组及米诺环素组(n=15)。分别采用免疫组织化学及免疫印迹法检测术后2周大鼠缺血侧海马GAP-43和PSD-95蛋白的表达,Morris水迷宫实验评价大鼠的行为学改变。结果:同假手术组相比,模型组大鼠缺血侧海马GAP-43(0.49±0.03)和PSD-95(0.92±0.04)表达明显增高(P=0.000);大鼠逃避潜伏期明显延长[(44.2±10.0)s],穿过原平台次数(2.6±0.9)和在目标象限探索时间百分率明显降低[(19.2±2.1)%,P=0.000)]。同模型组相比,尾静脉给予3 mg/kg米诺环素治疗后,大鼠缺血侧海马GAP-43(0.72±0.05)表达明显增加,PSD-95表达降低(0.67±0.05,P=0.000),大鼠逃避潜伏期明显缩短[(30.8±7.6)s,P=0.020]、穿过原平台次数(4.4±1.1,P=0.012)和在目标象限探索时间百分率明显增加[(30.4±2.7)%,P=0.000)]。结论:低剂量米诺环素能显著改善脑缺血再灌注后大鼠学习记忆能力,其机制可能与上调GAP-43和下调PSD-95表达有关。
[Abstract]:Aim: to investigate the effects of low dose minocycline on cognitive impairment and the expression of hippocampal growth-associated protein -43 associated protein-43 (GAP-43) and postsynaptic postsynaptic density 95 (PSD-95) in rats with focal cerebral ischemia-reperfusion injury.Methods: the middle cerebral artery occlusion reperfusion model of Sprague-Dawley rats was established by thread embolization. 45 male rats were randomly divided into sham-operation group, model group and minocycline group.The expressions of GAP-43 and PSD-95 protein in hippocampus of ischemic rats were detected by immunohistochemistry and Western blotting at 2 weeks after operation. The behavioral changes of rats were evaluated by Morris water maze test.缁撴灉:鍚屽亣鎵嬫湳缁勭浉姣，

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