超高效液相色谱串联质谱法测定人血浆中克拉霉素缓释片的浓度及其药动学

发布时间：2018-04-05 06:23

本文选题：克拉霉素缓释片　切入点：超高效液相色谱-串联质谱法　出处：《中国医院药学杂志》2017年13期

【摘要】：目的:建立高效、灵敏、快速的超高效液相色谱-电解质-质谱/质谱(UPLC-ESI-MS/MS)测定人血浆中克拉霉素浓度的方法,并应用于药动学研究。方法:血浆样品先经碳酸钠溶液碱化,经乙醚液-液萃取后,以0.05 mol·L~(-1)乙酸铵水溶液-乙腈为流动相进行梯度洗脱,色谱柱采用BEH C_(18)(50 mm×2.1 mm,1.7μm)色谱柱,克拉霉素和内标罗红霉素定量分析的离子对分别为m/z748.6→157.9和m/z837.0→679.2。结果:克拉霉素的线性范围为1~3 000 ng·mL~(-1)且线性关系良好,日内日间精密度均小于10.0%,准确度在-7.6%~6.0%之间,样品运行时间为2.0min。结论:该法灵敏、快速,适用于克拉霉素高通量样本的分析,已成功应用于克拉霉素人体药动学研究。
[Abstract]:Objective: to establish a high performance, sensitive and rapid method for the determination of clarithromycin in human plasma by UPLC-ESI-MS / MS.Methods: plasma samples were first alkalized by sodium carbonate solution, then extracted by ether liquid-liquid, then gradient elution was carried out with 0.05 mol ammonium acetate aqueous solution and acetonitrile as mobile phase. The chromatographic column was BEH C20 18mm 脳 2.1 mm ~ (1.7 渭 m) column.The ion pairs of clarithromycin and roxithromycin were m/z748.6 157.9 and m/z837.0 679.2, respectively.Results: the linear range of clarithromycin was 1 ~ 3 000 ng / mL 路L ~ (-1) and the linear relationship was good. The intra-day precision of clarithromycin was less than 10.0, the accuracy was between -7.6% and 6.0%, and the sample running time was 2.0 min.Conclusion: this method is sensitive, rapid and suitable for the analysis of clarithromycin in high throughput samples, and has been successfully applied to the pharmacokinetics of clarithromycin in human body.
【作者单位】：中国医科大学附属第一医院药学部;中国医科大学药学院;
【基金】：国家自然基金青年科学基金(编号:81302841) 辽宁省高等学校优秀人才支持计划(编号:LJQ2014086)
【分类号】：R969.1

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