转铁蛋白标记磁性脂质体的制备及体外成像

发布时间：2018-04-07 14:51

本文选题：脂质体　切入点：受体　出处：《中国组织工程研究》2017年06期

【摘要】：背景:利用转铁蛋白受体表达的特异性和转铁蛋白-转铁蛋白受体结合的亲和性,转铁蛋白修饰药物载体可以明显提高药物的特异结合能力和疗效。转铁蛋白也可修饰影像对比剂,针对肿瘤组织的转铁蛋白受体,实现肿瘤的特异性靶向成像。目的:评价转铁蛋白-磁性纳米粒的理化特性,并对其细胞结合特性进行体外实验研究。方法:采用旋转蒸发法制备磁性脂质体,共价结合法制备转铁蛋白-磁性纳米粒。对转铁蛋白-磁性纳米粒的粒径、电位、形态、转铁蛋白结合效率、r2弛豫率、细胞结合特性及细胞毒性等指标进行分析评价。转铁蛋白-磁性纳米粒与转铁蛋白受体高表达Hep G2肝癌细胞特异性结合后进行体外MR成像。结果与结论:1转铁蛋白-磁性纳米粒平均粒径为95.1 nm,多分散系数为0.21,Zeta电位为-1.25 m V,r2弛豫率为94.62 mmol~(-1)·s~(-1),每个磁性脂质体约结合27个转铁蛋白分子;2荧光共聚焦电镜显示转铁蛋白-磁性纳米粒与转铁蛋白受体高表达Hep G2肝癌细胞发生特异结合,荧光胞浆出现罗丹明红染;普鲁士蓝铁染色显示Hep G2细胞内和细胞表面有铁染色颗粒;3体外细胞MR T2WI成像显示,转铁蛋白-磁性纳米粒与Hep G2细胞标记后,离心管信号强度下降;而空白磁性纳米粒与细胞不发生结合,故无明显信号强度下降;4细胞毒性实验显示细胞生长曲线良好;5以上结果表明,转铁蛋白-磁性纳米粒满足MR分子探针的所需要求,可用于MR分子成像。
[Abstract]:Background: based on the specificity of transferrin receptor expression and the affinity of transferin-transferrin receptor binding, transferrin modified drug carrier can significantly improve the drug specific binding ability and efficacy.Transferrin can also be modified as a contrast agent for the specific target imaging of tumors.Aim: to evaluate the physical and chemical properties of transferrin-magnetic nanoparticles and to study their cell-binding properties in vitro.Methods: magnetic liposomes were prepared by rotating evaporation, and transferrin-magnetic nanoparticles were prepared by covalent binding method.The particle size, potential, morphology, r2 relaxation rate, cell binding properties and cytotoxicity of transferin-magnetic nanoparticles were analyzed and evaluated.In vitro Mr imaging was performed after specific binding of transferrin-magnetic nanoparticles to Hep G2 hepatoma cells overexpression of transferrin receptor.Results and conclusion the average particle size of 1: 1 transferin-magnetic nanoparticles was 95.1 nm, and the polydispersity coefficient was 0.21 渭 g Zeta potential -1.25 m V ~ (1) V ~ (-1) V ~ (2) V ~ (2) Li ~ (-1) 路s ~ (-1). Each magnetic liposome was bound to 27 transferrin molecules. The fluorescence confocal electron microscopy showed that each magnetic liposome was bound to 27 transferrin molecules, and the confocal electron microscopy showed that the relaxation rate was 94.62 mmol / L ~ (-1).Transferrin-magnetic nanoparticles specifically bind to Hep G2 hepatoma cells overexpression of transferrin receptor.Rhodamine red staining appeared in the fluorescent cytoplasm, Prussian blue iron staining showed that there were iron stained particles in Hep G2 cells and on the surface of the cells, and in vitro Mr T2WI images showed that transferrin magnetic nanoparticles were labeled with Hep G2 cells.The signal intensity of the centrifuge tube decreased, but the blank magnetic nanoparticles did not bind to the cells, so there was no obvious signal intensity decrease. The results showed that the cell growth curve was good.Transferrin-magnetic nanoparticles meet the needs of Mr molecular probes and can be used for Mr molecular imaging.
【作者单位】：广州中医药大学第二附属医院影像科;广州中医药大学第二附属医院药学部;
【基金】：广东省科技计划项目(2013B021800247) 国家自然科学基金青年项目(30700184)~~
【分类号】：R943

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