血管紧张素Ⅱ对足细胞葡萄糖转运蛋白1的表达及膜转位的影响

发布时间：2018-04-07 16:35

本文选题：足细胞　切入点：葡萄糖转运蛋白　出处：《中山大学学报(医学科学版)》2015年02期

【摘要】：【目的】观察血管紧张素II(Ang II)对体外培养的小鼠足细胞葡萄糖转运蛋白1(GLUT1)的表达及膜转位的影响。【方法】Ang II对GLUT1m RNA和GLUT1蛋白表达的检测将体外培养的足细胞分为4组:对照组(C)、低剂量组(Ang II 10-10mol/L)、中剂量组(Ang II 10-8 mol/L)及高剂量组(Ang II 10-6 mol/L),用RT-PCR、Western blot检测GLUT1m RNA和GLUT1蛋白的表达;Ang II对GLUT1足细胞胞膜分布检测也将足细胞分为4组:C组、胰岛素(Ins)10-6 mol/L组、Ang II 10-6 mol/L+Ins10-6mol/L组及氯沙坦(LO)10-6 mol/L+Ang II 10-6 mol/L+Ins 10-6 mol/L组,Western blot检测GLUT1在胞膜的蛋白表达及间接免疫荧光观察GLUT1在胞膜的分布。【结果】m RNA与蛋白表达分组中,与对照组相比,Ang II低、中、高浓度3组GLUT1m RNA水平分别增加了5.89%、21.46%、30.95%(P皆0.01),各组之间差别亦有统计学意义(P0.01);蛋白表达水平分别增加了8.40%、11.79%、20.57%(P皆0.01),各组之间差别亦有统计学意义(P0.01),Spearman相关分析显示Ang II浓度与GLUT1蛋白表达呈正相关(r=0.934,P0.001)。足细胞胞膜分布检测中,与对照组相比,足细胞胞膜GLUT1蛋白表达Ins 10-6 mol/L组增加了16.33%(P0.01)、Ang II 10-6 mol/L+Ins 10-6 mol/L组增加了3.27%(P0.05),LO 10-6 mol/L+Ang II 10-6mol/L+Ins 10-6mol/L组增加了11.33%(P0.01);免疫荧光显示Ins组GLUT1在胞膜上的分布明显多于对照组及Ang II+Ins组,而LO干预后,胞膜的荧光强度增加。【结论】Ang II能剂量依赖性地增加足细胞胞内GLUT1的表达及合成;Ang II能够阻碍胰岛素诱发的GLUT1向足细胞胞膜的膜转位,氯沙坦可部分阻断Ang II的作用。
[Abstract]:[objective] to observe the effect of angiotensin II (II(Ang II) on the expression of glucose transporter 1 (GLUT1) and membrane translocation of mouse podocyte in vitro. [methods] the expression of GLUT1m RNA and GLUT1 protein in podocytes cultured in vitro was detected by Ang II.Compared with the control group, Ang II was low and medium.The expression of GLUT1 protein was positively correlated with the expression of GLUT1 protein.In the detection of cell membrane distribution of podocytes, compared with the control group,[conclusion] Ang II can increase the expression of GLUT1 in podocyte in a dose-dependent manner and the synthesis of Angii can block the insulin-induced translocation of GLUT1 to the membrane of podocyte membrane. Losartan can partially block the effect of Ang II.
【作者单位】：广州医科大学附属第二医院肾内科;广州市第一人民医院肾内科;广州市第一人民医院儿科;
【基金】：广东省自然科学基金(S2011010003081)
【分类号】：R96

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