转铁蛋白修饰紫杉醇脂质体的制备及其抑瘤作用

发布时间：2018-04-10 14:19

本文选题：转铁蛋白 + 紫杉醇　；参考：《中国药房》2016年01期

【摘要】：目的:制备转铁蛋白(TF)修饰紫杉醇(PTX)脂质体(TF-PTX-LP),并研究其抑瘤作用。方法:采用薄膜分散法制备TF-PTX-LP并观察其形态;定性和定量考察肝癌Hep G2细胞对TF-LP和LP的摄取情况;检测PTX、PTX-LP和TF-PTX-LP分别处理Hep G2细胞24、48、72 h后细胞的增殖抑制率;以Hep G2细胞制备肿瘤球,考察生理盐水、PTX、PTX-LP和TF-PTX-LP分别处理0、1、2、4、5、6、7 d对肿瘤球体积的影响;复制Hep G2荷瘤裸鼠模型,考察生理盐水、PTX、PTX-LP和TF-PTX-LP(以PTX计8.5mg/kg)对荷瘤裸鼠移植瘤的抑制作用。结果:TF-PTX-LP均呈均一球形,粒径在100~120 nm。TF-LP处理后细胞的荧光强度强于LP(P0.01)。与PTX、PTX-LP比较,TF-PTX-LP处理后细胞的增殖抑制率更高(P0.01)。与生理盐水、PTX、PTX-LP比较,TF-PTX-LP处理后肿瘤球体积更小,对荷瘤裸鼠的抑瘤率更高,且6、7 d后抑瘤率差异有统计学意义(P0.01)。增殖抑制率和肿瘤球体积变化呈时间依赖性。结论:成功制得具有良好抑瘤作用的TF-PTX-LP。
[Abstract]:Aim: to prepare TF-PTX-LPX liposome modified with transferrin (TFT) and to study its antitumor effect.Methods: TF-PTX-LP was prepared by membrane dispersion method and its morphology was observed. The uptake of TF-LP and LP in Hep G2 cells of liver cancer was investigated qualitatively and quantitatively. The proliferation inhibition rate of Hep G2 cells treated with PTX PTX-LP and TF-PTX-LP for 24 48 h was detected.Results the fluorescence intensity of TF-PTX-LP cells was higher than that of LPP-0.01g cells treated with 100 ~ 120 nm.TF-LP.The proliferation inhibition rate of TF-PTX-LP was higher than that of PTX-LP.Compared with normal saline PTXX PTX-LP, TF-PTX-LP treated with TF-PTX-LP was smaller in volume and higher in tumor inhibition rate on nude mice bearing tumor, and there was significant difference in tumor inhibition rate between six and seven days after treatment with TF-PTX-LP.The inhibition rate of proliferation and the volume change of tumor balls were in a time dependent manner.Conclusion: TF-PTX-LPhad been successfully prepared.
【作者单位】：新乡医学院第一附属医院肿瘤科;新乡医学院第一附属医院神经外科;
【基金】：河南省医学科技攻关计划项目(No.2011020091)
【分类号】：R943;R96

【相似文献】