甜菜对胆碱缺乏诱导高同型半胱氨酸血症大鼠的影响

发布时间：2018-04-11 22:11

本文选题：甜菜碱 + 同型半胱氨酸　；参考：《卫生研究》2015年02期

【摘要】：目的探讨富含甜菜碱的甜菜对由胆碱缺乏导致的大鼠高同型半胱氨酸血症的抑制效果。方法健康Wistar大鼠共48只随机分为6组:对照组25%大豆分离蛋白(25S);胆碱缺乏组(25SCD);25SCD+0.05%甜菜碱(Bet)组;25SCD+0.10%Bet组;25SCD+4.12%甜菜(Beet)组和25SCD+8.24%Beet组。实验喂养10天后处死动物,测定血浆和肝脏中同型半胱氨酸(Hcy)、半胱氨酸等氨基酸指标以及高半胱氨酸甜菜碱甲基转移酶(BHMT)和胱硫醚β-合酶(CBS)等酶学指标。结果胆碱缺乏使血浆Hcy的浓度从(11.8±0.4)μmol/L(对照组25S)显著升高到(33.2±0.6)μmol/L(25SCD),P0.05。0.10%甜菜碱和8.24%甜菜的添加显著降低了血浆Hcy,并且呈现明显的剂量依赖性(P0.05)。甜菜添加对BHMT活性的改善效果已明显高于对照组。结论饲料中添加富含甜菜碱的甜菜促进了大鼠体内同型半胱氨酸的再甲基化和胱硫醚的合成,显著抑制了由胆碱缺乏诱导的高同型半胱氨酸血症的发生。
[Abstract]:Objective to investigate the inhibitory effect of betaine-rich beet on hyperhomocysteinemia induced by choline deficiency in rats.Methods A total of 48 healthy Wistar rats were randomly divided into 6 groups: control group (25% soybean protein isolate), choline deficiency group (25SCD) 25SCD 0.05% betaine betaine group, 25SCD 0.10t group (25SCD 4.12% beet) and 25SCD 8.24et group.After 10 days of feeding, the animals were killed and the levels of homocysteine, cysteine and other amino acids in plasma and liver, as well as homocysteine betaine methyltransferase (BHMT) and cystathionine 尾 -synthase (CBS) were determined.Results the concentration of plasma Hcy was significantly increased from 11.8 卤0.4 渭 mol / L (control 25s) to 33.2 卤0.6 渭 mol / L (P 0.05.0.10% betaine and 8.24% sugar beet).The effect of beet supplementation on BHMT activity was significantly higher than that of control group.Conclusion the addition of betaine in diet promoted homocysteine demethylation and cysteine synthesis in rats, and inhibited the occurrence of hyperhomocysteinemia induced by choline deficiency.
【作者单位】：中国疾病预防控制中心营养与健康所;日本静冈大学应用生物化学系;
【基金】：中国营养学会营养科研基金-帝斯曼专项科研基金(No.2014-059) 日本学术振兴会(JSPS)基础研究(C)(No.19580135)
【分类号】：R965

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