右美托咪定预处理对缺血再灌注大鼠心肌细胞的保护作用

发布时间：2018-04-11 22:41

  本文选题：缺血再灌注 + 右美托咪定　； 参考：《中国临床药理学杂志》2017年20期

【摘要】：目的研究右美托咪定(Dex)预处理对缺血再灌注大鼠心肌细胞凋亡及凋亡蛋白表达的影响。方法按照体重将雄性SD大鼠随机分为4组,每组7只:低高2个剂量实验组(结扎前0.5 h分别腹腔注射Dex 10,30μg·kg~(-1)),模型组及假手术组(结扎前0.5 h均腹腔注射0.9%Na Cl 5 m L·kg~(-1))。用结扎左冠状动脉前支构建心肌缺血-再灌注损伤大鼠模型。以缺口末端标记(TUNEL)法检测各组大鼠心肌细胞凋亡率;以免疫组化法检测各组大鼠心肌组织中Bcl-2及Bax蛋白表达情况。结果假手术组、模型组与低高2个剂量实验组的心肌细胞凋亡率(AR,%)分别为0.42±0.06,19.73±4.12,13.31±3.65,10.04±2.71;这4组的心肌组织Bcl-2阳性表达指数(PEI)分别为0.25±0.03,5.74±1.06,11.44±2.01,14.71±2.13;这4组的心肌组织Bax PEI分别为0.27±0.04,19.95±5.01,15.02±4.82,12.13±3.14;这4组的Bcl-2/Bax分别为0.93±0.05,0.29±0.04,0.76±0.06,1.20±0.07。与假手术组比较,模型组大鼠心肌细胞AR、Bcl-2、Bax PEI及Bcl-2/Bax的差异均有统计学意义(均P0.01);与模型组比较,低高2个剂量实验组大鼠心肌组织这4项指标差异均有统计学意义(均P0.01)。结论 Dex可通过上调Bcl-2蛋白表达及下调Bax蛋白表达,抑制心肌缺血-再灌注损伤大鼠心肌细胞的凋亡。
[Abstract]:Aim to study the effects of dexmetomidine Dexpreconditioning on myocardial apoptosis and apoptotic protein expression in rats with ischemia reperfusion.Methods male Sprague-Dawley rats were randomly divided into 4 groups according to their body weight. The rats in each group were divided into four groups: the low and high dose groups (Dex 1030 渭 g / kg) were injected intraperitoneally at 0.5 h before ligation, and the model group and sham-operation group (0.5 h before ligation) were injected intraperitoneally with 0.9%Na Cl 5 m L 路kg ~ (-1).The rat model of myocardial ischemia reperfusion injury was established by ligating the anterior branch of left coronary artery.The apoptosis rate of cardiac myocytes in each group was detected by Tunel Nick end labeling and the expression of Bcl-2 and Bax protein in myocardium of each group was detected by immunohistochemical method.缁撴灉鍋囨墜鏈�缁�,妯″瀷缁勪笌浣庨珮2涓�鍓傞噺瀹為獙缁勭殑蹇冭倢缁嗚優鍑嬩骸鐜�(AR,%)鍒嗗埆涓，

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