红树林耐酸真菌Aspergillus sp.OUCMDZ-1914次生代谢产物研究

发布时间：2018-04-14 15:13

  本文选题：红树林真菌 + 天然产物　； 参考：《有机化学》2015年09期

【摘要】：为获得活性微生物代谢产物,从海南文昌红树林根泥样品中分离筛选到耐酸真菌Aspergillus sp.OUCMDZ-1914,通过酸性条件发酵培养,发酵产物经过硅胶柱色谱、凝胶柱色谱和半制备高效液相色谱进行分离纯化,运用NMR、MS、UV、IR、X射线单晶衍射等手段鉴定了9个化合物的化学结构,即:(2Z,3E,5E,7E,9E)-4,10-二甲基-11-[3-(2,5-二氧亚基四氢吡咯)基]-2-(2-羟基乙亚基)-11-氧亚基-3,5,7,9-十一碳四烯酸甲酯(1)、RKB-3384A(2)、JBIR-74(3)、JBIR-75(4)、secalonic acid D(5)、oxaline(6)、meleagrin(7)、okaramine H(8)、citreorosein(9),其中1为新化合物.活性测试表明化合物2对甲型流感病毒H1N1的抑制活性与阳性药利巴韦林相当,IC50为116.2μmol/L;化合物5对A549、MCF-7和K562细胞株表现出较好的抑制活性,IC50分别0.23,4.45和3.68μmol/L;化合物6具有α-糖苷酶抑制活性,IC50为0.84mmol/L,与阳性药阿卡波糖相当.
[Abstract]:In order to obtain the active microbial metabolites, the acid-resistant fungus Aspergillus sp. OUCMDZ-1914 was isolated from the root mud samples of mangroves in Wenchang, Hainan, and cultured under acidic conditions. The fermentation products were separated by silica gel column chromatography.Gel column chromatography and semi-preparative high performance liquid chromatography were used to separate and purify the chemical structures of 9 compounds.鍗，

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