棒曲霉毒素对人胚肾细胞增殖与诱导其凋亡的研究

发布时间：2018-04-15 18:41

本文选题：棒曲霉素 + 细胞增殖　；参考：《现代食品科技》2015年03期

【摘要】：本文研究了棒曲霉素抑制人胚肾细胞增殖与诱导其凋亡的作用。不同浓度的棒曲霉素作用于人胚肾细胞293后,采用乳酸脱氢酶释放检测棒曲霉素对细胞增殖的抑制作用。通过扫描电镜和荧光显微镜观察了细胞形态学的变化,Annexin V-EGFP/PI标记检测细胞凋亡率,JC-1线粒体膜电位荧光探针检测了细胞线粒体膜电位的变化。实时荧光定量PCR测定了线粒体相关基因FIS1、ASL、SLC25A6、COX17的表达水平。结果表明,棒曲霉素可显著抑制人胚肾细胞293增殖并诱导凋亡,具有明显的量效关系,2.5、5、7.5、10和15μM棒曲霉素对人胚肾细胞293的抑制率分别为8.1%、18.2%、31.0%、42.2%和63.1%;5μM棒曲霉素处理0、3、10、24 h,细胞的存活率分别为94.2%、78.1%、47.4%。5μM和10μM的棒曲霉素处理8h后,细胞凋亡率分别为16.4%,和20.1%。线粒体膜电位和线粒体相关基因的表达水平的变化表明棒曲霉素可能通过线粒体途径引起人胚肾细胞293凋亡。
[Abstract]:The inhibitory effect of patulin on proliferation and apoptosis of human embryonic kidney cells was studied.After different concentrations of patulin were treated on 293 human embryonic kidney cells, the inhibitory effect of patulin on cell proliferation was detected by lactate dehydrogenase release.The changes of cell morphology were observed by scanning electron microscope and fluorescence microscope. The apoptosis rate was detected by Annexin V-EGFP/PI labeling and the change of mitochondrial membrane potential was detected by JC-1 mitochondrial membrane potential fluorescence probe.The expression of mitochondrial related gene ASIS1, ASLC25A6, was determined by real-time fluorescence quantitative PCR.The results showed that patulin could significantly inhibit the proliferation of human embryonic kidney cells 293 and induce apoptosis.The inhibitory rates of 2. 5 渭 M and 15 渭 M of patulin on human embryonic kidney cell line 293 were 8.1 and 18.21.0%, 42.2% and 63.1 渭 M, respectively. The cell survival rate was 94.28.47.4.5 渭 M and 10 渭 M respectively for 8 h, and the apoptosis rate was 16.4and 20.1um, respectively.The changes of mitochondrial membrane potential and mitochondrial related gene expression suggest that patulin may induce apoptosis of human embryonic kidney cells 293 through mitochondrial pathway.
【作者单位】：西北农林科技大学食品科学与工程学院;兰州理工大学生命科学与工程学院;
【基金】：国家自然基金(31201286) 中央高校基本科研创新一般项目(2014YB021)
【分类号】：R96

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