宁夏枸杞内生菌的抗菌和抗肿瘤活性研究

发布时间：2018-04-17 01:16

本文选题：枸杞 + 内生菌　；参考：《中国中药杂志》2017年11期

【摘要】：为筛选具有抗菌抗肿瘤活性的枸杞内生菌资源,对分离自宁夏枸杞的10株内生菌发酵液的抑菌活性和体外细胞毒活性进行检测,通过ITS和16S rDNA序列分析对菌株进行了分子鉴定。结果表明,5株内生真菌对供试病原菌都未表现出抑制活性,放线菌AL6对3种病原真菌都有一定的抑制作用,而AL5仅对金黄色葡萄球菌有较强抑制活性。但内生真菌的抗肿瘤活性明显强于放线菌。当发酵液浓度为0.2 g·L~(-1)时,有4株内生真菌至少对1种肿瘤细胞的抑制率高于50%。序列分析表明,5株内生真菌隶属于曲霉属、青霉属和翘孢霉属。5株内生放线菌隶属于链霉菌属、微球菌属和拟诺卡氏菌属。曲霉属菌株FL1对A549和HeLa细胞的抑制活性都较强,其IC_(50)分别为0.022,0.028 g·L~(-1),值得进一步研究。
[Abstract]:In order to screen endophytic resources of Lycium barbarum with antibacterial and antitumor activities, the antibacterial activity and cytotoxic activity of 10 strains of endophytic bacteria from Ningxia Lycium barbarum were detected. The strains were identified by ITS and 16s rDNA sequence analysis.The results showed that 5 strains of endophytic fungi had no inhibitory activity against the pathogens tested, while actinomycetes AL6 had a certain inhibitory effect on the three pathogenic fungi, while AL5 only had strong inhibitory activity on Staphylococcus aureus.But the antitumor activity of endophytic fungi was obviously stronger than that of actinomycetes.When the fermentation broth concentration was 0.2 g / L ~ (-1), the inhibition rate of 4 strains of endophytic fungi on at least one tumor cell was higher than 50%.Sequence analysis showed that 5 endophytic fungi belong to Aspergillus, and 5 endophytic actinomycetes belong to Streptomyces, Micrococcus and Nocardia.The inhibitory activity of Aspergillus strain FL1 on A549 and HeLa cells was strong, and its IC _ (50) was 0.022 ~ 0.028 g / L ~ (-1), respectively, which was worthy of further study.
【作者单位】：宁夏大学生命科学学院;
【基金】：宁夏自然科学基金项目(NZ1601)
【分类号】：R915

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