黄原胶对前交叉韧带切断术所致骨关节炎的疗效及其机制

发布时间：2018-04-18 13:46

本文选题：黄原胶 + 骨关节炎　；参考：《药学学报》2017年10期

【摘要】：本研究评价黄原胶(xanthan gum,XG)对前交叉韧带切断术(anterior cruciate ligament transaction,ACLT)所致的兔膝关节骨关节炎(osteoarthritis,OA)模型中软骨损伤的保护作用,并探讨XG对OA软骨中caspase-3、Bax蛋白表达的影响。将60只雄性新西兰大耳白兔按随机数字表法分为6组,每组10只,随机选取1组为正常对照组(control组),剩余5组右膝采用ACLT建立OA模型,并根据药物干预不同分为模型组(model组)、XG-0.6 mg·k~(g-1)、XG-1.2 mg·k~(g-1)、XG-2.4 mg·k~(g-1)治疗组和玻璃酸钠(sodium hyaluronate,SH-1.2 mg·k~(g-1))治疗组。测量治疗过程中各组兔膝关节局部温度和膝关节宽度;治疗结束后,对各组兔关节进行大体形态学观察;通过HE染色对各组兔关节软骨组织病理学形态进行观察;Western blot法检测各组家兔软骨细胞中Bax和caspase-3的激活态cleaved caspase-3含量。实验结果表明,XG可抑制OA所致的膝关节局部温度的上升和膝关节宽度的增加,且具有剂量依赖性;XG具有改善由OA所致的股骨髁和胫骨平台形态学异常和组织损伤的作用;Western blot结果表明:与control组相比,model组和XG-0.6 mg·k~(g-1)组家兔膝关节软骨细胞中Bax和cleaved caspase-3水平显著增加(P0.01),model组和XG-0.6 mg·k~(g-1)组间兔膝关节软骨细胞中Bax和cleaved caspase-3水平无显著性差别(P0.05),且明显高于XG-1.2 mg·k~(g-1)和XG-2.4 mg·k~(g-1)组(P0.01),XG-2.4 mg·k~(g-1)和XG-1.2 mg·k~(g-1)组间兔骨关节软骨细胞中cleaved caspase-3水平无显著性差别(P0.05),XG-2.4 mg·k~(g-1)组兔骨关节软骨细胞中Bax水平低于XG-1.2 mg·k~(g-1)组(P0.05)。综上所述,XG能够有效地保护OA中的软骨损伤,并能够抑制OA软骨中Bax和caspase-3蛋白的表达。
[Abstract]:The purpose of this study was to evaluate the protective effect of xanthan gumma XG on cartilage injury induced by anterior cruciate ligament transaction (ACLT) in rabbits with osteoarthritis of knee joint, and to investigate the effect of XG on the expression of caspase-3Bax protein in OA cartilage.Sixty male New Zealand white rabbits were randomly divided into 6 groups with 10 rabbits in each group. One group was randomly selected as control group, and the remaining 5 groups were treated with ACLT to establish OA model.According to the drug intervention, the model group was divided into two groups: model group (XG-0.6 mg / kg) and sodium hyaluronate sodium hyaluronateSH-1.2 mg / kg ~ (-1) (treated with XG-1.2 mg / kg ~ (-1) + XG-2.4mg / kg ~ (-1)) respectively, and the model group was treated with sodium hyaluronate SH-1.2 mg / kg ~ (-1) sodium hyaluronate (SH-1.2 mg / kg ~ (-1)).The local temperature and width of knee joint in each group were measured during the treatment, and the gross morphology of each group was observed after the treatment.The histopathological morphology of articular cartilage in each group was observed by HE staining. The contents of Bax and caspase-3 active cleaved caspase-3 in chondrocytes of each group were detected by Western blot method.The experimental results showed that XG could inhibit the rise of knee joint temperature and the increase of knee joint width induced by OA.The results of Western blot showed that compared with control group and XG-0.6 mg / kg -1 group, Bax and cleaved in articular chondrocytes of rabbit knee joint were improved.There was no significant difference in the levels of Bax and cleaved caspase-3 in rabbit knee joint chondrocytes between the two groups (P 0.05) and XG-2.4 mg / kg ~ (1) and XG-2.4 (mg / kg ~ (-1)) respectively. There was no significant difference in cleaved caspase-3 level in articular chondrocytes between the two groups (P _ (0.01) and XG-0.6 mg / kg ~ (-1)) and XG-1.2 (mg / kg ~ (1)) groups. (P _ (0.05)) and XG-2.4 (mg / kg ~ (-1)), respectively, there was no significant difference in cleaved caspase-3 level in articular chondrocytes between the two groups (P _ (0.01) and XG-1.2 mg / kg ~ (-1)).The level of Bax in rabbit osteoarticular chondrocytes was lower than that in XG-1.2 group (P 0.05).In conclusion, XG can effectively protect cartilage injury and inhibit the expression of Bax and caspase-3 in OA cartilage.
【作者单位】：锦州医科大学药学院;山东省药学科学院生物制药重点实验室多糖药物工程实验室多糖药物国家-地方联合工程实验室;中国人民解放军93263部队医院;锦州医科大学附属第一医院;
【基金】：国家自然科学基金资助项目(81302681) 国家“十二五”重大新药创制基金资助项目(2012ZX093016-002) 辽宁省自然科学基金资助项目(2013022051) 辽宁省大学生创新训练项目(201510160000004,201510160000016)
【分类号】：R965

【相似文献】