埃罗替尼对小鼠角膜上皮组织病理学及超微结构的影响

发布时间：2018-04-20 13:19

本文选题：埃罗替尼 + 角膜上皮　；参考：《眼科新进展》2017年05期

【摘要】：目的探讨埃罗替尼对小鼠角膜上皮组织引起的组织病理学及超微结构改变的影响。方法30只雄性6~8周龄BALB/c小鼠,随机分为对照组(12只)和实验组(12只),6只不作处理为空白对照。实验组使用20μmol·L~(-1)埃罗替尼液滴眼,对照组采用PBS滴眼,每天4次。分别在干预后1 d、7 d、14 d对各组小鼠进行荧光素钠(fluorescent,FL)染色及评分。干预后14 d取小鼠眼球,光学显微镜及电子显微镜下观察角膜上皮及细胞的结构变化。提取角膜蛋白进行Western Blot检测。结果干预前,实验组和对照组FL评分比较差异无统计学意义(P0.05)。在干预后1 d、7 d、14 d,实验组FL评分较干预前明显升高,差异均有统计学意义(均为P0.05),而对照组FL评分与干预前无明显改变,差异均无统计学意义(均为P0.05);两组间FL评分在干预后1 d相比,差异无统计学意义(P0.05);两组间FL评分在干预后7 d、14 d相比,差异均有统计学意义(均为P0.05)。实验组小鼠角膜上皮细胞排列紊乱,层数增加;电镜下见角膜上皮表层细胞形态不规则、脱落,微绒毛减少、消失;表层上皮细胞的桥粒、半桥粒连接数目明显减少。实验组角膜表皮生长因子受体表达明显发生变化,两组表皮生长因子受体比较差异有统计学意义(P0.05)。结论埃罗替尼会引起小鼠角膜上皮的组织结构及细胞超微结构损坏。其机制可能是通过抑制表皮生长因子受体的活化来影响角膜上皮的。
[Abstract]:Objective to investigate the effects of erotinib on histopathological and ultrastructural changes induced by corneal epithelium in mice. Methods Thirty male BALB/c mice aged 6 to 8 weeks were randomly divided into control group (n = 12) and experimental group (n = 12). The experimental group was treated with 20 渭 mol L ~ (-1) Erotini drops, while the control group was treated with PBS drops 4 times a day. The mice in each group were stained with sodium fluorescein fluorescein FLL on the 7th day and 14th day after intervention. The cornea epithelium and cell structure were observed by optical microscope and electron microscope 14 days after intervention. Cornea protein was extracted for Western Blot detection. Results before intervention, there was no significant difference in FL score between the experimental group and the control group (P 0.05). The scores of FL in the experimental group were significantly higher than those before the intervention on the 1st day, 7th day and 14th day after the intervention (all P 0.05), but the FL score of the control group had no significant change compared with that before the intervention. There was no significant difference in FL score between the two groups (P 0.05), and there was no significant difference in FL score between the two groups on the 1st day after intervention (P 0.05), and there was significant difference in FL score between the two groups at 7 d and 14 d after intervention (all P 0.05). In the experimental group, the corneal epithelial cells arranged disorderly and the number of layers increased; under the electron microscope, the corneal epithelial surface cells were irregular in shape, shedding off, the microvilli decreased and disappeared; the desmosome and semi-desmosome junctions of the surface epithelial cells decreased significantly. The expression of epidermal growth factor receptor (EGF) in the experimental group was obviously changed, and the difference between the two groups was statistically significant (P 0.05). Conclusion Erotini can damage the tissue structure and cell ultrastructure of corneal epithelium in mice. The mechanism may affect corneal epithelium by inhibiting the activation of epidermal growth factor receptor (EGF).
【作者单位】：西安市胸科医院病理科;陕西省眼科诊疗中心西安市第四医院眼科西安交通大学医学院附属广仁医院眼科;厦门大学眼科研究所;西安交通大学第一附属医院病理科;南昌大学第一附属医院眼科;
【基金】：国家自然科学基金资助(编号:81660158、81160118、81400424、81460092、81400372) 江西省远航工程(编号:2014022) 江西省自然科学基金重大项目(编号:2016ACB21017);江西省自然科学基金(编号:20151BAB215016) 江西省科技支撑计划项目(编号:20151BBG70223) 江西省卫计委面上项目(编号:20175116) 陕西省创新人才推进计划项目(2017) 陕西省科学技术研究发展计划项目(编号:2014K11-03-07-04)~~
【分类号】：R965

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