阿托伐他汀对老年自身免疫性脑脊髓膜炎大鼠脑白质的保护作用

发布时间：2018-04-21 13:02

本文选题：阿托伐他汀 + 自身免疫性脑脊髓膜炎　；参考：《中国老年学杂志》2015年19期

【摘要】：目的探讨阿托伐他汀对老年自身免疫性脑脊髓膜炎(EAE)大鼠脑白质髓鞘碱性蛋白(MBP)和少突胶质细胞转录因子(OLIG)1蛋白表达的影响。方法取老年Wistar雄性大鼠80只,随机分为对照组、模型组、阿托伐他汀大剂量组(8 mg/kg组)和阿托伐他汀小剂量组(2 mg/kg组),每组20只。采用新鲜豚鼠50%全脊髓匀浆液和完全弗氏佐剂(CFA)免疫制备EAE模型,免疫后第2天开始,模型组给予生理盐水灌胃,阿托伐他汀组给予阿托伐他汀不同剂量灌胃,持续灌胃21 d,于第22天处死,取脑干和脊髓待测。采用实时荧光定量和免疫组织化学染色检测各组脑干、脊髓中MBP和OLIG1的mRNA水平和蛋白阳性表达强度。结果与对照组比较,模型组MBP mRNA水平和蛋白阳性表达强度明显升高、OLIG1明显降低(P0.01);阿托伐他汀小剂量组MBP mRNA水平和蛋白阳性表达强度升高、OLIG1降低(P0.01);阿托伐他汀大剂量组MBP mRNA水平和蛋白阳性表达强度略升高、OLIG1略降低,无显著差异(P0.05),模型组、阿托伐他汀大、小剂量组差异显著(P0.01)。结论阿托伐他汀能够通过下调MBP、上调OLIG1 mRNA水平和蛋白阳性表达强度,对EAE大鼠的脑白质髓鞘损伤具有保护作用,且大剂量作用优于小剂量。
[Abstract]:Objective to investigate the effects of atorvastatin on the expression of white myelin basic protein (MBP) and oligodendrocyte transcription factor (OLIG) 1 protein (OLIG) 1 protein in aged autoimmune cerebrospinal meningitis. Methods 80 aged Wistar male rats were randomly divided into the control group, the model group, the large dose of atorvastatin group (8 mg/kg group) and atorvastatin. The small dose group (2 mg/kg group), 20 in each group, was immunized with 50% whole spinal cord homogenate and complete Freund's adjuvant (CFA) to prepare the EAE model. After second days of immunization, the model group was given the normal saline for gastric perfusion. The Atorvastatin group was given the Atorvastatin at different doses of the stomach, and the gastric perfusion was 21 d, and the brain stem and spinal cord were tested for the brain stem and spinal cord. The mRNA level and protein positive expression intensity of MBP and OLIG1 in the brain stem and spinal cord were detected by real time fluorescence quantitative and immunohistochemical staining. Results compared with the control group, the level of MBP mRNA and protein positive expression increased significantly in the model group, and the OLIG1 decreased significantly (P0.01), and the level of MBP mRNA and protein Yang in the small dose atorvastatin group were compared with those in the control group. The intensity of sexual expression increased and OLIG1 decreased (P0.01); the level of MBP mRNA and protein positive expression in the large dose atorvastatin group increased slightly, the OLIG1 decreased slightly (P0.05), the model group, the Atorvastatin large and the small dose group were significantly different (P0.01). Conclusion atorvastatin can increase the OLIG1 mRNA level and protein positive by down regulation of MBP. The expression intensity has protective effect on myelin sheath damage in EAE rats, and the effect of large dose is better than that of low dose.

【作者单位】：内蒙古医科大学附属医院神经内科;长春大学特教学院;内蒙古医科大学病理教研室;
【基金】：内蒙古自治区自然科学基金(No.2011MS1154)
【分类号】：R965

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