P物质与香草酸瞬时受体亚型1在慢性非细菌性前列腺炎大鼠神经痛中的作用研究

发布时间：2018-04-22 03:26

本文选题：香草酸瞬时受体亚型 + P物质　；参考：《中华男科学杂志》2015年02期

【摘要】：目的:探讨慢性非细菌性前列腺炎(CNP)疼痛的可能机制。方法:自身免疫法建立CNP大鼠模型;Von Frey丝检测机械刺激缩足阈(PWT);进行前列腺病理学检查和免疫组化检测前列腺与脊髓L5~S2节段P物质(SP)、香草酸瞬时受体亚型1(TRPV1)表达变化和相关性。结果:造模后大鼠PWT[(48.83±11.55)g]下降,与对照组[(67.98±5.44)g]相比差异有统计学意义(P0.05)。前列腺呈现显著炎症反应,病变范围、间质淋巴细胞浸润都较对照组反应程度有所加重,差异有统计学意义(P0.05)。与对照组相比,模型组前列腺与脊髓背角L5~S2中SP、TRPV1阳性表达均上调,差异有统计学意义(P0.05)。前列腺与脊髓中SP蛋白表达无相关关系(r=0.099,P=0.338);TRPV1蛋白表达亦无相关关系(r=0.000,P=0.5)。结论:SP、TRPV1可能通过脊髓L5~S2节段的表达上调参与了CNP大鼠疼痛的形成和维持。
[Abstract]:Objective: To explore the possible mechanism of pain in chronic non bacterial prostatitis (CNP). Methods: CNP rat model was established by autoimmunization; Von Frey wire was used to detect the mechanical stimulation contraction threshold (PWT); prostate pathology examination and immunohistochemical detection of P substance (SP) in prostate and spinal cord L5~S2 segment, vanillic acid transient receptor subtype 1 (TRPV1) expression change Results: the PWT[(48.83 + 11.55) g] decreased after the model, compared with the control group [(67.98 + 5.44) g]), the difference was statistically significant (P0.05). The prostate showed significant inflammatory response, the lesion range and interstitial lymphocyte infiltration were more severe than the control group (P0.05). Compared with the control group, the model group was compared with the control group. The positive expression of SP and TRPV1 in the prostate and spinal dorsal horn L5~S2 all up up, the difference was statistically significant (P0.05). There was no correlation between the expression of SP protein in the prostate and the spinal cord (r=0.099, P=0.338), and there was no correlation between the expression of TRPV1 protein (r=0.000, P=0.5). Conclusion: SP, TRPV1 may be involved in the pain of rats through the up regulation of the segment of the spinal cord. Formation and maintenance.

【作者单位】：新疆医科大学第五附属医院病理科;新疆医科大学第五附属医院中医科;新疆医科大学第五附属医院院部;
【基金】：国家自然科学基金(81060321)~~
【分类号】：R965

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