肝素化磁介孔硅纳米粒负载多柔比星的体外抗癌活性研究

发布时间：2018-04-23 10:06

本文选题：肝素化 + 磁介孔硅纳米粒　；参考：《中国药学杂志》2015年24期

【摘要】：目的制备肝素化磁介孔硅纳米粒,并负载多柔比星,考察该载药系统的体外释药及抗癌活性。方法用相转移法和溶胶-凝胶法合成单分散核/壳结构的磁介孔硅纳米粒(MMSNs),以共价结合的方法合成肝素化磁介孔硅纳米粒,然后进行药物在肝素化磁介孔硅纳米粒上的负载与体外释放实验、肝素化磁介孔硅纳米粒的细胞摄入实验及肝素化磁介孔硅纳米粒负载多柔比星的体外细胞学实验。结果肝素化磁介孔硅纳米粒能够明显延缓多柔比星的释放,能够被肝癌细胞HepG2摄入、对HepG2细胞具有明显的杀伤作用、对碱性成纤维细胞生长因子诱导的HepG2细胞增殖具有明显的抑制作用。结论肝素化磁介孔硅纳米粒作为药物载体在传递抗肿瘤药物方面具有潜在的应用前景。
[Abstract]:Objective to prepare the heparin mesoporous silica nanoparticles and load doxorubicin, to investigate the in vitro release and anticancer activity of the drug delivery system. Methods the magnetic mesoporous Kong Gui nanoparticles (MMSNs) were synthesized by phase transfer and sol-gel method, and were covalently combined with the magnetic mesoporous silica nanoparticles of heparin. The load and in vitro release of heparinated magnetic mesoporous silicon nanoparticles, the cell uptake experiment of heparinated mesoporous silicon nanoparticles and the in vitro cytological test of the heparinated mesoporous silicon nanoparticles loaded doxorubicin. Results the heparinated magnetic mesopore Kong Gui nanoparticles could significantly delay the release of doxorubicin and be able to be absorbed by the hepatoma cell HepG2. It has obvious killing effect on HepG2 cells and obviously inhibits the proliferation of HepG2 cells induced by basic fibroblast growth factor. Conclusion heparin magnetic mesoporous silica nanoparticles as drug carriers have potential applications in the transfer of antitumor drugs.

【作者单位】：河南大学药学院;广东药学院;
【基金】：国家自然科学基金资助项目(21073054)
【分类号】：R943;R96

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