去甲斑蝥素联合紫杉醇对非依赖前列腺癌细胞的抑制作用

发布时间：2018-04-23 10:55

本文选题：去甲斑蝥素 + 紫杉醇　；参考：《中国临床药理学杂志》2017年15期

【摘要】：目的研究去甲斑蝥素(NCTD)、紫杉醇(PTX)及两者联合使用对前列腺癌细胞的抑制作用。方法实验分为3组:NCTD组、PTX组和联合组(NCTD+PTX)。NCTD组和PTX组:在处于指数增长的前列腺癌细胞株DU145中,加入不同浓度(12.5,25.0,50.0,100.0,200.0,400.0,800.0μmol·L~(-1))的NCTD和PTX;联合组:NCTD与PTX按1∶1浓度配比,重复第1组操作。48 h后再加入3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)工作液20μL,培养4 h后吸出上层液体,加入DMSO 200μL进行溶解。以离心培养法测定DU145的复苏率和计算细胞生长抑制率;以MTT法检测半数抑制浓度。以模拟联合指数考察两药物是否具有协同作用。结果上述由小到大7个浓度NCTD组对癌细胞的抑制率分别为0.12%,0.14%,0.25%,0.30%,0.58%,0.70%,0.74%,PTX组分别为0.42%,0.43%,0.44%,0.46%,0.46%,0.47%,0.52%,联合组分别为0.53%,0.55%,0.56%,0.61%,0.66%,0.75%,0.79%,联合组对癌细胞的抑制率高于NCTD组与PTX组,差异均有统计学意义(均P0.05)。随着两药剂量的增加,模拟联合指数值越来越高,且小于1,最低为0.07±0.01,最高为0.59±0.20,与最低浓度比较差异有统计学意义(P0.05),表明NCTD、PTX联用时,两者间有协同作用,且随着剂量的增加,协同作用更明显。结论 NCTD与PTX对非依赖前列腺癌细胞均有抑制效果,且浓度越高抑制作用越强;两者联合使用作用更强。
[Abstract]:Objective to study the inhibitory effects of norcantharidin (NCTD), paclitaxel (PTX) and their combination on prostate cancer cells. Methods PTX).NCTD and PTX groups were divided into 3 groups: NCTD and PTX with different concentrations of 12.5NCTD 25.0100.0200.0400.00.0800.0 渭 mol Lamp-1A were added to the prostate cancer cell line DU145 with exponential growth, and the concentration of NCTD and PTX was 1:1 in the combined group, the two groups were divided into two groups: the control group and the control group, the control group and the control group were divided into three groups, the control group and the control group were divided into three groups: NCTD and PTX were added to the prostatic cancer cell line DU145 with different concentrations. The operation of group 1 was repeated for 48h, and then the working liquid of 3-tetrothiazole-2-dimethylthiazole-2-diphenyltetrazolium bromine salt (MTT) was added to 20 渭 L, and the upper liquid was sucked out after 4 hours of culture, and then dissolved with DMSO 200 渭 L. The resuscitation rate and cell growth inhibition rate of DU145 were measured by centrifugation and half inhibitory concentration was measured by MTT method. The synergistic effect of the two drugs was investigated by a simulated combination index. With the increase of the amount of the two drugs, the simulated joint index value is higher and higher, and the lowest is 0.07 卤0.01, the lowest is 0.07 卤0.01, the highest is 0.59 卤0.20. The difference is statistically significant compared with the lowest concentration (P 0.05), which indicates that there is a synergistic effect between the two groups when combined with NCTDX, and with the increase of the dose, The synergy is more obvious. Conclusion both NCTD and PTX have inhibitory effects on non-dependent prostate cancer cells, and the higher the concentration is, the stronger the inhibitory effect is.
【作者单位】：潍坊市益都中心医院药学部;潍坊市益都中心医院院长办公室;潍坊市益都中心医院神经内科;潍坊市益都中心医院科教科;山东中医药大学基础学院;
【基金】：山东省自然科学基金青年基金资助项目(2014ZRB14193)
【分类号】：R96

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