氢化可的松角质类脂脂质体的理化性质及其体外经皮渗透研究

发布时间：2018-04-27 21:05

本文选题：氢化可的松 + 角质类脂脂质体　；参考：《中国新药杂志》2017年15期

【摘要】：目的:制备氢化可的松角质类脂脂质体,并考察其理化性质和体外透皮性能。方法:采用乙醇注入法制备氢化可的松角质类脂脂质体并通过透射电镜观察外观形态;通过葡聚糖凝胶微柱测定包封率;用激光散色法测定粒径和Zeta电位;以大鼠皮肤为渗透屏障,采用透皮扩散仪测定氢化可的松角质类脂脂质体的体外透皮累积渗透量和皮肤滞留量。结果:氢化可的松角质类脂脂质体粒径均匀且圆整,粒径为(433.90±30.71)nm,Zeta电位为(-33.09±1.86)m V,包封率为(40.32±1.56)%,p H值为(5.77±0.03);角质类脂脂质体的体外经皮渗透率为(10.87±0.76)μg·cm-2·h-1,为普通脂质体的1.91倍;12 h后皮肤中药物滞留量为(3.31±0.15)μg·cm-2,显著高于普通脂质体。结论:氢化可的松角质类脂脂质体制备工艺简单,可有效促进氢化可的松的经皮渗透,为氢化可的松经皮给药新制剂的研究提供了参考。
[Abstract]:Aim: to prepare hydrocortisone keratinoid liposomes and investigate their physical and chemical properties and transdermal properties in vitro. Methods: hydrocortisone keratinoid liposomes were prepared by ethanol injection and the appearance of liposomes was observed by transmission electron microscope. The encapsulation efficiency was measured by dextran gel microcolumn. The particle size and Zeta potential were measured by laser discoloration method. Rat skin was used as the permeation barrier. The cumulative penetration and skin retention of hydrocortisone keratinoid liposomes in vitro were measured by transdermal diffuser. Results: the diameter of hydrocortisone keratinoid liposomes was uniform and round. The particle size was 433.90 卤30.71 渭 g cm-2 h-1, and the entrapment efficiency was 40.32 卤1.56 渭 g cm-2 h-1, and the percutaneous permeability of keratinoid liposome was 10.87 卤0.76 渭 g cm-2 h-1, which was 3.31 卤0.15 渭 g cm-2 after 12 hours of normal liposome, which was significantly higher than that of common liposome. Conclusion: hydrocortisone keratinoid liposome is simple and can promote transdermal permeation of hydrocortisone.
【作者单位】：中国医科大学附属盛京医院药学部;武汉市妇女儿童医疗保健中心药学部;
【分类号】：R943

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