油脂类药用辅料橄榄油中甾醇谱的研究

发布时间：2018-04-27 21:45

  本文选题：药用辅料 + 橄榄油　； 参考：《药物分析杂志》2015年11期

【摘要】：目的:分析油脂类辅料橄榄油中甾醇组成及质量分数。方法:首先从橄榄油中提取不皂化物,运用高效液相色谱法从不皂化物中分离并收集甾醇的馏分。液相色谱条件:采用Kromasil 60-5 SIL硅胶柱(250 mm×4.6 mm,5μm),以异丙醇-正己烷(1∶99,v/v)为流动相等度洗脱,流速1.0 m L·min-1,检测波长210 nm,进样量20μL。然后对馏分进行衍生化,采用气相色谱法检测各甾醇的组成及百分含量;气相色谱条件:采用DB-5毛细管柱(30 m×0.25 mm×0.25μm),进样口温度:290℃;进样量:2~3μL;分流比为25∶1;载气:氦气;流速1.8 m L·min-1;程序升温:起始柱温260℃,保持50 min,再以5℃·min-1的速率升温至290℃,保持5 min。结果:橄榄油中含有多种甾醇,如胆甾醇、菜子甾醇;菜油甾醇、豆甾醇、△7-菜油甾醇、△5,23-豆甾二烯醇、赤桐甾醇、β-谷甾醇、谷甾烷醇、△5-燕麦甾醇、△5,24-豆甾二烯醇、△7-豆甾醇等,其中β-谷甾醇的含量比较高,占总甾醇量的80%以上,其次是菜油甾醇,△5,24-豆甾二烯醇等。结论:本法可以对油脂类辅料橄榄油中的甾醇谱进行分析,为油脂类药用辅料植物油中甾醇谱的研究提供了参考。
[Abstract]:Objective: to analyze the composition and mass fraction of sterol in olive oil. Methods: the unsaponifiable compounds were extracted from olive oil and sterol fractions were separated and collected by high performance liquid chromatography (HPLC). The liquid chromatographic conditions were as follows: Kromasil 60-5 SIL silica gel column (250mm 脳 4.6mm), isopropanol-n-hexane (1: 9: 9 v / v) as the flow equality elution, flow rate 1.0 mL min-1, detection wavelength 210nm, sample volume 20 渭 L. The composition and percentage content of sterols were determined by gas chromatography. The gas chromatographic conditions were as follows: DB-5 capillary column: 30m 脳 0.25 mm 脳 0.25 渭 m, sample temperature: 290 鈩，

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