小鼠体内气管滴注纳米二氧化硅对体内主要脏器的影响

发布时间：2018-05-02 05:17

本文选题：纳米二氧化硅 + 气管滴注　；参考：《吉林大学学报(医学版)》2017年02期

【摘要】：目的:探讨气管滴注纳米二氧化硅(SiO_2)在体内对小鼠各脏器的影响,为纳米SiO_2的安全性评估提供参考依据。方法:40只6~8周龄BALB/c雌鼠随机分为对照组(生理盐水)、低剂量SiO_2组(7mg·kg~(-1))、中剂量SiO_2组(21mg·kg~(-1))和高剂量SiO_2组(35mg·kg~(-1))(n=10),非暴露式气管滴注染毒5次,每3d1次,最后一次染毒1和15d后取小鼠左肺、右肾、肝脏、心脏和脾脏制成石蜡切片,苏木精-伊红(HE)染色后光镜下进行组织形态学检查;摘眼球取血,检测肝肾功能生化指标。结果 :与对照组比较,各实验组尤其是中和高剂量SiO_2组小鼠肺组织出现肺泡间隔增厚、炎性细胞浸润的炎症实变表现以及少量的小动脉血栓;肝脏出现肉芽肿样炎性细胞浸润及少量的局灶性肝细胞坏死;脾脏红髓增大充血,巨噬细胞增生并可见散在的巨核细胞;上述表现存在剂量依赖效应,随着时间的推移,15d后这些损伤表现均有减轻的趋势;肾组织则表现为轻微的炎性细胞浸润。血生化指标检测,丙氨酸氨基转移酶(ALT)与天冬氨酸氨基转移酶(AST)水平在实验组中有一定的波动,提示肝细胞有不同程度损伤;实验组小鼠尿素氮(BUN)和肌酐(Cr)水平变化提示有肾功能损害,但无明显剂量及时间效应。结论:气管滴注纳米SiO_2对不同脏器的影响主要表现在炎症及损伤方面,受影响的脏器主要有肺脏、肝脏、脾脏和肾脏。
[Abstract]:Objective: to investigate the effect of intratracheal instillation of nano-SiO2 / SiO2 on the viscera of mice, and to provide a reference for the safety evaluation of nano-SiO _ 2. Methods 40 female BALB/c rats of 6 and 8 weeks of age were randomly divided into two groups: control group (normal saline group, low dose SiO_2 group, 7 mg SiO_2 group, middle dose SiO_2 group, 21 mg SiO_2 group) and high dose SiO_2 group, 35 mg SiO_2 group and 35 mg BALB/c group, 5 times of unexposed tracheal drip, 5 times per 3d1, and 1 and 15 days after the last dose of SiO_2, the left lung of mice was harvested. The right kidney, liver, heart and spleen were made into paraffin sections. The histomorphology was examined under light microscope after hematoxylin-eosin (HEH) staining, blood was taken from eyeball and biochemical indexes of liver and kidney function were detected. Results: compared with the control group, the alveolar septum thickening, inflammatory cell infiltration and a small amount of arteriole thrombosis were found in the lung tissues of the experimental groups, especially in the middle and high dose SiO_2 groups. Liver granulomatous inflammatory cell infiltration and a small number of focal hepatocyte necrosis; splenic red pulp enlargement and congestion, macrophage proliferation and scattered megakaryocyte can be seen. With the passage of time, 15 days later, all of these lesions tended to decrease, while the renal tissues showed slight inflammatory cell infiltration. The levels of alanine aminotransferase (alt) and aspartate aminotransferase (AST) were fluctuated in the experimental group. The changes of bun (bun) and creatinine (Cr) levels in the experimental group indicated renal function damage, but there was no significant dose and time effect. Conclusion: the effects of intratracheal instillation of nanometer SiO_2 on different organs are mainly manifested in inflammation and injury. The affected organs mainly include lung, liver, spleen and kidney.
【作者单位】：首都医科大学公共卫生学院卫生毒理与卫生化学系;首都医科大学环境毒理学北京市重点实验室;
【基金】：国家自然科学基金资助课题(81402709)
【分类号】：R965

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