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超高效液相色谱法测定肝病患者氟康唑血药浓度

发布时间:2018-05-03 17:18

  本文选题:氟康唑 + 血药浓度 ; 参考:《中国医院药学杂志》2015年17期


【摘要】:目的:建立用超高效液相色谱法测定肝病患者血中氟康唑的浓度。方法:采用超高效液相色谱(UPLC)法,Zorbax Eclipse plus-C18柱(2.1 mm×100 mm,1.8μm),保护柱Zorbax SB-C18柱(2.1 mm×5 mm,1.8μm),以乙腈-水(24∶76)为流动相,在210 nm处测定其含量。结果:线性范围为0.1~20μg·ml-1,样品提取回收率为80.95%(n=5),方法回收率为98.67%~106.37%,日内精密度RSD≤4.21%,日间RSD≤2.17%,且稳定性良好。结论:该方法可靠、简便、快速,可为临床合理用药提供参考。
[Abstract]:Objective: to establish a high performance liquid chromatography method for the determination of fluconazole in the blood of patients with liver disease. Methods: Zorbax Eclipse plus-C18 column (2.1 mm 脳 100mm ~ 1.8 渭 m) and Zorbax SB-C18 column (2. 1 mm 脳 5 mm ~ 1.8 渭 m) were used as mobile phase and acetonitrile-water (24: 76) as mobile phase. The content was determined at 210nm. Results: the linear range was 0.1 ~ 20 渭 g ml ~ (-1). The recovery of the sample was 80.95%, the recovery rate was 98.67 ~ 106.37%, the precision of the day RSD 鈮,

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