天冬氨酸及赖氨酸2～10肽单链和双链的合成与毒性

发布时间：2018-05-04 23:37

本文选题：氨基酸 + 单链多肽　；参考：《解剖学报》2015年04期

【摘要】：目的探讨同种氨基酸形成多肽的毒性和应用前景。方法用固相合成法合成天冬氨酸和赖氨酸的2~10肽单链与双链化合物,通过小鼠尾静脉将这些化合物注射到小鼠肝脏内,观察它们对小鼠的致死作用(LD50)。结果当天冬氨酸单链2~10肽的浓度依次达到0.15、0.1、0.09、0.03、0.0065、0.03、0.034、0.035和0.04 mol/L时,小鼠的死亡率达到50%。当赖氨酸单链2~10肽的浓度依次达到0.28、0.1、0.047、0.0225、0.0028、0.00166、0.0015、0.0011和0.00075 mol/L时,小鼠的死亡率达到50%。当天冬氨酸-赖氨酸双链2~10肽的浓度依次得到0.5、0.05、0.017、0.014、0.009、0.006、0.004、0.004、0.004、0.004 mol/L时,小鼠的死亡率达到50%。结论单链多肽的毒性大于双链多肽。在2~6肽范围内,天冬氨酸单链多肽随着氨基酸数目增加毒性逐渐增加;相反,在7~10肽范围内,随着氨基酸数目的增加毒性逐渐降低。在2~10肽范围内,赖氨酸单链多肽随着氨基酸数目增加毒性逐渐增加。在2~6肽范围内,天冬氨酸-赖氨酸双链多肽随着氨基酸数目增加毒性逐渐增加;但在7~10肽范围内,其毒性相似。
[Abstract]:Objective to study the toxicity and application prospect of homologous amino acid polypeptide. Methods the single chain and double chain compounds of aspartic acid and lysine were synthesized by solid state synthesis. These compounds were injected into the liver of mice through tail vein to observe their lethal effect on mice. Results when the concentration of aspartic acid single-chain 2tiapeptide reached 0.150.1-0.09U 0.03U 0.0065U 0.03N 0.03N 0.035 and 0.04 mol/L, the mortality of mice reached 50%. When the concentration of lysine single chain 2o 10 peptide reached 0.28 ~ 0.41 ~ 0. 047 ~ 0. 022 ~ 25 ~ 0. 0028 ~ 0. 0016 ~ 6 ~ 0. 0015 ~ 0. 0011 and 0.00075 mol/L, the death rate of mice reached 50. When the concentration of aspartic acid-lysine double chain 2tiapeptide was obtained in turn, the death rate of mice reached 50% when the concentration of 0. 05 ~ 0. 05 ~ 0. 017 ~ 0. 017 ~ 0. 0017 ~ 0. 0014 ~ 0. 006 ~ 0. 006 ~ 0. 004 ~ 0. 004 ~ 0. 004 ~ 0. 004 mol/L. Conclusion the toxicity of single chain polypeptide is greater than that of double chain polypeptide. The toxicity of aspartic acid single chain polypeptide increased with the increase of amino acid number in the range of 2 ~ 6 peptide, but decreased with the increase of amino acid number in the range of 7 ~ 10 peptide. The toxicity of lysine single chain peptide increased with the increase of amino acid number in the range of 2 ~ 10 peptide. The toxicity of aspartic acid-lysine double chain peptide increased with the increase of amino acid number in the range of 2 ~ (6) peptide, but the toxicity was similar in the range of 7 ~ (10) peptide.
【作者单位】：河南师范大学化学化工学院;河南省-科技部共建细胞分化调控重点实验室培育基地和生物工程重点实验室;河南师范大学生命科学学院;
【基金】：河南省基础与前沿技术研究资助项目(132300413208,132300410134)
【分类号】：R965

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