中空纤维离心超滤法检测人血浆中氨茶碱的游离药物浓度

发布时间：2018-05-06 01:38

本文选题：氨茶碱 + 游离药物　；参考：《中国医院药学杂志》2015年17期

【摘要】：目的:建立一个准确、简单、快速的分析人血浆中游离氨茶碱浓度的方法,并应用于临床治疗药物监测。方法:0.5 ml血浆样品经过中空纤维离心超滤(HFCF-UF)处理,以甲醇-10 mmol·L-1乙酸铵缓冲溶液(15/85,V/V)为流动相,采用Diamonsil C18色谱柱分离,流速为1.0ml·min-1,检测波长为275 nm,进样量为20μl。结果:血浆中游离氨茶碱浓度在0.25~25μg·ml-1范围内线性关系良好,最低定量限0.1μg·ml-1,日内、日间RSD分别小于1.1%和1.3%。结论:该方法简单、准确、重现性好,特别适合基层开展临床游离药物浓度的监测。该方法成功的应用于临床游离氨茶碱浓度的监测,为临床游离氨茶碱治疗药物监测提供了一个可靠的分析平台和进一步阐述游离药物浓度与药物疗效或毒性之间的关系奠定了基础。
[Abstract]:Objective: to establish an accurate, simple and rapid method for the determination of free aminophylline in human plasma. Methods the plasma samples of 0.5 ml were treated by hollow fiber centrifugation ultrafiltration (HFCF-UFF). The mobile phase was methanol-10 mmol L-1 ammonium acetate buffer solution (15 / 85 V / V). The separation was performed on a Diamonsil C18 column with a flow rate of 1.0ml min-1. The detection wavelength was 275nm and the sample injection was 20 渭 l. Results: the concentration of free aminophylline in plasma was linear in the range of 0.25 渭 g ml-1, the lowest limit of quantification was 0.1 渭 g ml-1, and the daytime RSD was less than 1.1% and 1.3%, respectively. Conclusion: this method is simple, accurate and reproducible, especially suitable for clinical free drug concentration monitoring. The method has been successfully applied to the monitoring of clinical free aminophylline concentration. It provides a reliable analysis platform for clinical free aminophylline therapeutic drug monitoring and further expounds the relationship between free drug concentration and drug efficacy or toxicity.
【作者单位】：中国石油天然气集团公司中心医院药学部;河北医科大学药学院;
【分类号】：R969

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