斑马鱼肿瘤异种移植模型在抗癌药敏感性评价中的应用

发布时间：2018-05-07 19:44

本文选题：斑马鱼 + 肿瘤移植　；参考：《中国比较医学杂志》2017年11期

【摘要】：目的应用人类肺癌、胃癌和肝癌细胞斑马鱼异种移植模型,分别评价5种临床常用抗癌药的体内敏感性。方法分别建立斑马鱼肺癌A549、胃癌SGC-7901和肝癌Hep G2异种移植模型,顺铂、紫杉醇、长春瑞滨、恩度和贝伐单抗设计3个剂量分别处理斑马鱼肺癌A549移植模型,紫杉醇、伊立替康、羟基脲、顺铂和5-氟尿嘧啶(5-fluorouracil,5-FU)设计3个浓度或剂量分别处理斑马鱼胃癌SGC-7901移植模型,阿霉素、吉西他滨、羟基脲、顺铂和5-氟尿嘧啶设计3个浓度或剂量分别处理斑马鱼肝癌Hep G2移植模型。实验结束后,使用荧光显微镜进行体内肿瘤定量图像分析,计算药物对斑马鱼体内肿瘤生长抑制率,通过与模型对照组相比,分析是否具有统计学意义。结果受试的抗肿瘤药物在斑马鱼肿瘤异种移植模型中均有效,并且基本都呈剂量依赖性。在抗肺癌A549药敏试验中,药效从高到低分别为贝伐单抗(65%)顺铂(55%)长春瑞滨(40%)恩度(39%)紫杉醇(27%);在抗胃癌SGC-7901药敏试验中,药效从高到底分别为羟基脲(46%)5-FU(31%)=伊立替康(31%)紫杉醇(26%)顺铂(24%);在抗肝癌Hep G2药敏试验中,药效从高到底分别为顺铂(64%)羟基脲(56%)吉西他滨(46%)阿霉素(45%)5-FU(38%)。结论斑马鱼肿瘤异种移植模型适合用于抗癌药体内药敏试验。
[Abstract]:Objective to evaluate the in vivo sensitivity of five commonly used anticancer drugs in human lung cancer, gastric cancer and liver cancer cell xenotransplantation models of zebrafish. Methods the xenotransplantation models of A549, SGC-7901 and Hep G2 of zebrafish lung cancer were established, and three doses of cisplatin, paclitaxel, vinorelbine, Endor and bevacizumab were designed to treat A549, paclitaxel, irinotecan respectively. Hydroxyurea, cisplatin and 5-fluorouraciline 5-FU (5-fluorouraciline 5-FU) were designed to treat SGC-7901 transplanted model of zebrafish gastric cancer, adriamycin, gemcitabine, hydroxyurea, doxorubicin, gemcitabine, hydroxyurea respectively. Three doses of cisplatin and 5-fluorouracil were designed to treat the Hep G2 transplantation model of liver cancer in zebrafish. At the end of the experiment, quantitative image analysis of tumor in vivo was carried out with fluorescence microscope to calculate the inhibitory rate of drug on tumor growth of zebrafish. Compared with the model control group, the analysis was statistically significant. Results the antitumor drugs were effective in zebrafish xenotransplantation model, and were basically dose-dependent. In the drug sensitivity test of anti-lung cancer A549, the drug efficacy from high to low were bevacizumab 65) cisplatin 55) vinorelbine 4010) Endox 39) paclitaxel 27g; in the anti-gastric cancer SGC-7901 susceptibility test, The pharmacodynamics from the high to the end is: 5-FU31and 5-FU31and 5-FU31a) cisplatin and cisplatin; in the drug sensitivity test of anti-hepatoma Hep G2, the drug effect is from high to cisplatin 64) hydroxyurea 56-1) gemcitabine 4646) doxorubicin 4545 and 5-FU38. Conclusion the xenotransplantation model of zebrafish tumor is suitable for drug sensitivity test in vivo.
【作者单位】：杭州环特生物科技股份有限公司;浙江省人用物品安全性评价技术研究重点实验室早期评价基地;浙江省医学科学院安全性评价研究中心;
【基金】：浙江省科技重大专项(编号:2014C03009)
【分类号】：R-332;R965

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