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新型可控性栓塞剂MNPs-tTF的构建

发布时间:2018-05-08 23:00

  本文选题:磁流体 + 截短组织因子 ; 参考:《中国生物工程杂志》2017年09期


【摘要】:目的:构建新型的具有可控性且能诱发动物血栓的栓塞剂——磁靶向凝血蛋白,从而为动物血栓模型的建立提供新工具。方法:使用化学共沉淀法合成磁流体(magnetic nanoparticles,MNPs),通过透析对其纯化后用超导量子干涉磁强针(SQUID)测定其磁性能;利用基因工程方法表达截短组织因子(truncated tissue factor,tTF)(又称为凝血蛋白)并纯化与鉴定;采用戊二醛交联法构建磁靶向凝血蛋白(MNPs-tTF),体外分析其磁控靶向性及凝血效力。结果:成功构建了磁靶向凝血蛋白MNPs-tTF,体外实验证实其保留有磁流体MNPs的磁控靶向性,以及凝血蛋白tTF的凝血效力。结论:成功构建了磁靶向凝血蛋白MNPs-tTF,为血栓模型动物的建立提供一种新工具。
[Abstract]:Aim: to construct a new type of chemoembolization agent, magnetic target coagulation protein (MTAP), which can induce thrombus in animals, so as to provide a new tool for the establishment of animal thrombus model. Methods: MNPs were synthesized by chemical coprecipitation method and their magnetic properties were determined by means of superconducting quantum interference magnetic intensity needle (SQUID) after dialysis. The truncated tissue factor (truncated tissue factor) was expressed, purified and identified by genetic engineering, and the magnetic target coagulation protein (MNPs-tTFA) was constructed by glutaraldehyde crosslinking method, and its magnetic targeting and coagulation potency were analyzed in vitro. Results: the magnetically targeted coagulation protein MNPs-tTFs was successfully constructed. It was confirmed by experiments in vitro that the MNPs-tTFs retain the magnetically controlled targeting of MNPs and the coagulation potency of tTF. Conclusion: MNPs-tTFs were successfully constructed, which provides a new tool for the establishment of thrombotic model animals.
【作者单位】: 厦门大学抗癌研究中心;解放军第174医院厦门大学附属成功医院肿瘤科;厦门大学实验动物中心;
【基金】:福建省科技计划重点项目(2014Y0082) 福建省属公益类项目(2015R1036-3,2016R1034-2)资助项目
【分类号】:R943

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