环磷酰胺对小鼠免疫抑制作用过程中代谢通路变化的代谢组学研究

发布时间：2018-05-10 20:32

  本文选题：环磷酰胺 + 免疫抑制　； 参考：《中国实验动物学报》2017年05期

【摘要】：目的采用代谢组学方法,研究环磷酰胺对小鼠免疫抑制作用过程中代谢通路的变化。方法采用流式细胞术了解免疫细胞变化情况,通过建立偏最小二乘法模型多结果进行判别。以偏最小二乘法模型中变量重要性投影(variable importance in projection,VIP)参数筛选潜在的生物标志物,通过统计分析确定差异性代谢物,借助代谢组学小分子化合物快速鉴定分析软件系统对差异性代谢物进行进一步鉴定分析,通过搜库及运算最终确定差异代谢物质9种,通路富集结果分析差异代谢通路3条。结果环磷酰胺对正常机体的不饱和脂肪酸的生物合成、线粒体脂肪酸代谢、甘油磷脂代谢、甾类激素的生物合成、花生四烯酸代谢、脂肪酸代谢、嘧啶的生物合成等代谢通路均产生明显影响。结论环磷酰胺在正常组与实验组中影响最重要的代谢通路为花生四烯酸代谢、甘油磷脂代谢和嘧啶代谢。
[Abstract]:Objective to study the changes of metabolic pathway during immunosuppressive effect of cyclophosphamide in mice. Methods flow cytometry was used to investigate the changes of immune cells. The potential biomarkers were screened by the variable importance in the partial least square model, and the different metabolites were determined by statistical analysis. The differential metabolites were further identified and analyzed with the help of the rapid identification and analysis software system of metabolomics small molecular compounds. Nine different metabolites were identified by searching database and operation, and three different metabolic pathways were analyzed by the enrichment results of pathway. Results the biosynthesis of unsaturated fatty acids, mitochondrial fatty acid metabolism, glycerol phospholipid metabolism, steroid hormone biosynthesis, arachidonic acid metabolism and fatty acid metabolism were observed. Pyrimidine biosynthesis and other metabolic pathways have a significant effect. Conclusion the most important metabolic pathway of cyclophosphamide in normal group and experimental group is arachidonic acid metabolism, glycerol phospholipid metabolism and pyrimidine metabolism.
【作者单位】： 重庆市中药研究院;
【基金】：重庆市科委应用开发(重大)项目(cstc2014yykf C10004);(cstc2014yykf C10005);重庆市科委基本业务费(2016cstc-jbky-01912);重庆市科委社会事业与民生保障科技创新专项(cstc2017shms A130090;cstc2017shms A130035)
【分类号】：R965
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本文编号：1870764