单采猪血浆的质量研究

发布时间：2018-05-10 22:32

本文选题：单采猪血浆 + 总蛋白　；参考：《科学技术与工程》2017年07期

【摘要】：研究单采猪血浆的质量情况,为建立猪血浆的质量标准提供参考依据。参照中国药典、欧洲药典及猪源性生物制品相关要求检测血浆的外观、pH、渗透压、总蛋白含量、枸橼酸盐含量、钠钾钙离子含量、是否无菌以及对猪细小病毒进行PCR检测方法的建立与应用。结果显示单采血浆外观正常,渗透压、总蛋白含量、枸橼酸盐含量、钾离子、钙离子、钠离子分别为315±34 mOsnlol/kg、53.7±3.2 g/L、15.98±4.88 mmol/L、3.00±0.45 mmol/L、2.35±0.38 mmol/L、151.3±22.4 mmol/L,检测结果均符合人血浆制品或者人血浆标准,但pH 7.81±0.21,其与人血浆规定含量稍有差异。单采血浆样品中只有1个样品检出细菌阳性。建立了猪细小病毒特异敏感的PCR检测方法,检测结果显示单采猪血浆猪细小病毒阳性率为0。实验为单采猪血浆质量基本符合现有人血浆或血浆制品质量标准。
[Abstract]:To study the quality of single-collected pig plasma and to provide reference for establishing the quality standard of pig plasma. According to the requirements of Chinese Pharmacopoeia, European Pharmacopoeia and Porcine Biological products, the plasma pH, osmotic pressure, total protein content, citrate content, sodium, potassium and calcium ion content were determined. Establishment and application of PCR detection method for porcine parvovirus. The results showed that the plasma was normal in appearance, osmotic pressure, total protein content, citrate content, potassium ion, calcium ion and sodium ion were 315 卤34mOsnlol / kg ~ (-1) 53.7 卤3.2 mg / L ~ (-1) ~ (15.98 卤4.88) mmol / L ~ (-1) 3.00 卤0.45 mmol / L ~ (2.35 卤0.38) mmol / L ~ (151.3 卤22.4) mmol 路L ~ (-1), respectively. But pH was 7.81 卤0.21, which was slightly different from that of human plasma. Only one of the plasma samples was positive for bacteria. A specific and sensitive PCR detection method for porcine parvovirus was established. The results showed that the positive rate of porcine parvovirus was 0. The results showed that the quality of pig plasma met the existing quality standard of human plasma or plasma products.
【作者单位】：南方医科大学;广州军区广州总医院药剂科;广东药科大学;广州市众为生物技术有限公司;
【基金】：广州市科技计划(201509010012) 国家重大新药创制项目(2013ZX09J13110-02B)资助
【分类号】：R927

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