近红外光介导的尺寸可逆转变超声造影剂的制备及其性质

发布时间：2018-05-13 22:25

  本文选题：超声造影剂 + 体外超声成像　； 参考：《药学学报》2017年09期

【摘要】：制备一种纳米级的载药超声造影剂,该纳米粒可有效累积分布至肿瘤深层部位,且体外实验证明,在升高温度或近红外光辐照下可实现纳米粒尺寸的纳米-微米转变:评价其体外粒径变化规律、体外超声显像效果及体内分布情况。所制备的纳米粒呈球形,脂质膜中可见中空金纳米球(hollow gold nanoparticle,HAu NS)的负载,粒径为302±5 nm,分散性指数为0.195±0.018,分布较均匀。在近红外光辐照(1或2 W·cm~(-2))下导致含有该纳米粒的溶液(以HAu NS计,0.2、0.04和0.02 g·L~(-1))升温迅速,利用激光粒度仪的控温功能,发现升温至52℃左右时即可检测到一定量的微米级粒子产生;在体外超声显像实验中,纳米粒经近红外光辐照后超声诊断仪检测到大量上扬的微泡,可增强体外超声显像的效果;体内分布实验表明,该纳米粒可因肿瘤的高渗透及滞留效应(EPR效应)在肿瘤中大量累积。本研究制备了可在肿瘤内部大量累积的纳米-微米可逆转变的超声显像纳米粒,为实现肿瘤病灶部位的超声显像同时结合光热-化学治疗提供了一种可行的方案。
[Abstract]:A kind of nano-scale drug-loaded ultrasound contrast agent was prepared, which can effectively accumulate and distribute to the deep part of the tumor, and has been proved by experiments in vitro. The nanometer-micron transition of nanoparticles can be realized at elevated temperature or near infrared irradiation. The change of particle size in vitro, the effect of in vitro ultrasound imaging and the distribution in vivo are evaluated. The prepared nanoparticles were spherical, and the hollow gold nanospheres hollow gold nanoparticlehau NSN could be seen in the lipid membrane. The particle size was 302 卤5 nm, the dispersion index was 0.195 卤0.018, and the distribution was uniform. The solution containing the nanoparticles (0.2n 0.04 and 0.02g / L ~ (-1) ~ (-1) was induced by near-infrared irradiation (1 or 2 W / cm ~ (-1). By using the temperature control function of the laser particle size meter, it was found that a certain amount of micron particles could be detected when the temperature was raised to about 52 鈩，

本文编号：1885099