黄连-左氧氟沙星联合用药对多重耐药大肠埃希菌的体外抗菌活性研究

发布时间：2018-05-14 19:36

本文选题：黄连提取物 + 左氧氟沙星　；参考：《中国医院药学杂志》2017年05期

【摘要】：目的:研究左氧氟沙星与黄连中成分联合用药对多重耐药大肠埃希菌的体外抑制作用。方法:选择从临床标本中检出的多重耐药大肠埃希菌,分别测定黄连提取物、盐酸小檗碱、盐酸巴马汀及左氧氟沙星对多重耐药大肠埃希菌最小抑菌浓度(MIC),采用微量肉汤棋盘稀释法分别测定黄连提取物、盐酸小檗碱、盐酸巴马汀与左氧氟沙星的体外联合抑菌作用。结果:实验结果表明黄连提取物、盐酸小檗碱、盐酸巴马汀与左氧氟沙星联合用药后对大部分耐药菌(90.9%)均表现为协同或相加作用,其中盐酸巴马汀与左氧氟沙星的联合应用最有效。结论:盐酸巴马汀、盐酸小檗碱或黄连提取物分别与左氧氟沙星联合对多重耐药大肠埃希菌都有较好的体外抗菌作用,临床应用价值高,值得进一步研究。
[Abstract]:Aim: to study the inhibitory effect of levofloxacin combined with Coptis chinensis on multidrug resistant Escherichia coli in vitro. Methods: the multidrug resistant Escherichia coli isolated from clinical specimens was selected and the extracts of Coptis chinensis and berberine hydrochloride were determined respectively. The minimal inhibitory concentration of palmatine hydrochloride and levofloxacin against multidrug resistant Escherichia coli was determined by chessboard dilution method. The combined bacteriostatic effects of Coptis chinensis extract, berberine hydrochloride, palmatine hydrochloride and levofloxacin in vitro were determined by chessboard dilution method. Results: the results showed that the extract of Rhizoma Coptidis, berberine hydrochloride, palmatine hydrochloride and levofloxacin were synergistic or additive to most resistant bacteria. The combination of palmatine hydrochloride and levofloxacin is the most effective. Conclusion: the combination of palmatine hydrochloride, berberine hydrochloride or Coptis chinensis extract with levofloxacin has a good antibacterial effect on multidrug resistant Escherichia coli in vitro, which is worthy of further study.
【作者单位】：九江学院附属医院检验科;九江学院药学与生命科学学院省级重点实验中心;
【基金】：江西省卫生厅课题(九卫科教字〔2013〕19号:20143244)
【分类号】：R96

【相似文献】