厄贝沙坦对大鼠梗死心肌组织中HGF mRNA和蛋白水平的影响

发布时间：2018-05-14 18:00

  本文选题：HGF + 厄贝沙坦　； 参考：《中国病理生理杂志》2017年06期

【摘要】：目的:通过观察厄贝沙坦对大鼠心肌梗死模型中缺血心肌肝细胞生长因子(HGF)mRNA和蛋白水平的影响,探讨厄贝沙坦减轻心肌纤维化的可能机制。方法:采用雄性Wistar大鼠建立心肌梗死模型,术后24 h将存活大鼠随机分为模型组和厄贝沙坦组,另设假手术组,每组9只大鼠。厄贝沙坦组予以厄贝沙坦50 mg·kg-1·d-1灌胃,模型组及假手术组给予等体积生理盐水灌胃,每天1次。4周后心脏取材,测量各组大鼠体重及左室重量,HE染色法观察缺血心肌病理改变,RT-q PCR和Western blot法检测各组大鼠心肌组织中HGF mRNA及蛋白水平的表达。结果:HE染色可见假手术组心肌细胞排列整齐,模型组及厄贝沙坦组心肌结构紊乱,药物组病理改变较模型组减轻;4周后各组大鼠体重无显著差异,各组间左心室重量差异有统计学意义(P0.01),厄贝沙坦组及模型组左室重量高于假手术组(P0.05),同时厄贝沙坦组低于模型组(P0.05);各组大鼠心肌组织中均检测到HGF的mRNA及蛋白水平,厄贝沙坦组及模型组HGF的mRNA与蛋白含量均高于假手术组(P0.05),厄贝沙坦组HGF的mRNA与蛋白水平低于模型组(P0.05)。结论:心肌梗死模型大鼠应用厄贝沙坦治疗4周时左室重量明显减轻,心肌组织病理变化得到改善,心肌组织中HGF的mRNA与蛋白水平降低。
[Abstract]:Aim: to investigate the effect of irbesartan on the mRNA and protein levels of hepatocyte growth factor (HGFN) in ischemic myocardium of rats with myocardial infarction, and to explore the possible mechanism of irbesartan in alleviating myocardial fibrosis. Methods: male Wistar rats were used to establish myocardial infarction model. The surviving rats were randomly divided into model group and irbesartan group 24 hours after operation. Irbesartan group was given irbesartan 50 mg kg-1 d-1 intragastrically, model group and sham operation group were given the same volume of normal saline. The pathological changes of ischemic myocardium were observed by HE staining and RT-Q PCR and Western blot were used to detect the expression of HGF mRNA and protein in myocardial tissue of rats in each group. Results the myocardial cells were arranged neatly in the sham-operation group, and the myocardial structure in the model group and irbesartan group were disordered. The pathological changes in the drug group were not significantly different from those in the model group after 4 weeks. The left ventricular weight of irbesartan group and model group was higher than that of sham-operated group (P 0.05), and irbesartan group was lower than model group (P 0.05). The mRNA and protein levels of HGF were detected in myocardium of all groups. The contents of mRNA and protein in HGF of irbesartan group and model group were higher than those of sham operation group (P 0.05), and the mRNA and protein levels of HGF in irbesartan group were lower than those in model group (P 0.05). Conclusion: after treatment with irbesartan for 4 weeks, left ventricular weight was significantly reduced, myocardial histopathological changes were improved, and the mRNA and protein levels of HGF in myocardial tissue were decreased in myocardial infarction model rats.
【作者单位】： 哈尔滨医科大学附属第四医院老年病科一病房;
【基金】：黑龙江省自然科学基金资助项目(No.H201389)
【分类号】：R965
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本文编号：1888920