醋甲唑胺干预对急进高原大鼠的损伤保护作用

发布时间：2018-05-15 23:11

本文选题：急进高原 + 醋甲唑胺　；参考：《中国药学杂志》2015年19期

【摘要】：目的探究急进4 300 m高原对Wistar大鼠机体血气生化改变、病理变化、血清肿瘤坏死因子-α和白细胞介素-1β影响及醋甲唑胺干预对大鼠高原损伤的保护作用。方法将28只成年健康Wistar大鼠按体重随机分为平原(海拔55米)对照组、高原(海拔4 300 m)模型组、醋甲唑胺组、乙酰唑胺组,分别灌胃给予0.9%氯化钠注射液、醋甲唑胺(每日2次,剂量为2.23 mg·kg-1)和乙酰唑胺(每日2次,剂量为22.33 mg·kg-1),对大鼠进行生化、血气分析、病理比较,并检测所得血清样品肿瘤坏死因子-α和白细胞介素-1β含量。结果血气、生化和病理结果显示,高原组大鼠出现脱水症状,与平原对照组比较,高原模型组各指标变化显著(P0.01),与高原模型组比较,醋甲唑胺组和乙酰唑胺组门冬氨酸转氨酶(AST)、丙氨酸氨基转移酶(ALT)、酸碱度(p H)、碳酸氢根离子浓度、缓冲碱(BB)、剩余碱(BE)等指标显著下降(P0.01),总蛋白(TP)、尿素(UREA)、二氧化碳分压(Pa CO2)、钠离子浓度、氯离子浓度等指标显著上升(P0.01),表明两组均出现代谢性酸中毒合并呼吸性碱中毒,且肝肺组织出现病理性损伤,其中,与乙酰唑胺组比较,醋甲唑胺组损伤较小。与平原对照组相比,高原组血清肿瘤坏死因子-α显著性升高,白细胞介素-1β显著性降低,其中,乙酰唑胺组和醋甲唑胺组血清肿瘤坏死因子-α、白细胞介素-1β含量均显著高于模型组(P0.01)。结论醋甲唑胺能改善急进高原大鼠的生理生化状况,减轻炎症损伤,具有较好的缺氧保护作用。
[Abstract]:Objective to investigate the changes of blood gas biochemistry, pathological changes, serum tumor necrosis factor- 伪 (TNF- 伪) and interleukin-1 尾 (IL-1- 尾) in Wistar rats at a rapid altitude of 4 300m and the protective effect of alacetazolamine on high altitude injury in rats. Methods 28 healthy adult Wistar rats were randomly divided into plain (55 meters above sea level) control group, high altitude (4 300 m) model group, acetozolamide group and acetazolamide group by intragastric administration of 0.9% sodium chloride injection. The contents of tumor necrosis factor- 伪 and interleukin-1 尾 in serum samples of rats were determined by biochemical analysis, blood gas analysis, pathological comparison, and acetazolamide (2.23 mg 路kg ~ (-1) and acetazolamide (22.33 mg 路kg ~ (-1), 2 times daily. Results the blood gas, biochemical and pathological results showed that the rats in the plateau group had dehydration symptoms. Compared with the plain control group, the indexes of the high altitude model group changed significantly (P 0.01), and those of the plateau model group were higher than those of the plateau model group. Acetazolamide and acetazolamide groups aspartate aminotransferase, alanine aminotransferase, acidazolamide group and acetazolamide group, aspartate transaminase, alanine aminotransferase, alkalinity, hydrogen carbonate ion concentration, The indexes such as buffered base BBN, residual alkaloid (BEB), total protein TPN, urea urEAA, carbon dioxide partial pressure, sodium ion concentration and chloride ion concentration increased significantly (P 0.01), which indicated that metabolic acidosis combined with respiratory alkalosis occurred in both groups. Pathological injury was found in liver and lung tissue, and the injury was less in acetazolamide group than in acetazolamide group. Compared with plain control group, serum tumor necrosis factor- 伪 (TNF- 伪) and interleukin-1 尾 (IL-1- 尾) in high altitude group were significantly increased. The serum levels of tumor necrosis factor- 伪 and interleukin-1 尾 in acetazolamide group and methazolamide group were significantly higher than those in model group (P 0.01). Conclusion Acetazolamide can improve the physiological and biochemical status and reduce the inflammatory injury of rats. It has a better protective effect on hypoxia.
【作者单位】：兰州军区兰州总医院全军高原环境损伤防治重点实验室;武汉市普仁医院;
【基金】：国家科技部重大资助项目(2008ZXJ09014-010) 全军医药科研“十二五”重点资助项目(BWS12J012) 全军医药科研“十二五”面上项目(CWS11C231)
【分类号】：R965

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