基因表达谱技术分析棒曲霉毒素肾细胞毒性的机制

发布时间：2018-05-19 18:56

本文选题：棒曲霉毒素 + 数字基因表达谱　；参考：《现代食品科技》2017年06期

【摘要】：本文研究了食品污染物棒曲霉毒素诱导人胚肾细胞损伤过程中基因表达谱动态变化。5μM PAT作用HEK293细胞3、24h,应用数字基因表达谱(DGE)技术筛选差异表达基因,分析基因富集的GO功能,KEGG信号通路及Pathway信号途径分析,并通过定量PCR对部分差异表达基因进行验证。分析发现,在3 h和24 h受PAT诱导的差异表达基因分别为79个和261个,其中上调表达基因分别为71和192个,下调表达基因分别为8和69个。PAT主要诱导了细胞凋亡基因的差异表达,RNA降解差异基因全部表达上调,RNA合成差异基因表达下调,泛素介导的蛋白降解大部分差异基因上调,MAPK信号通路主要参与PAT毒性的下游进程,其相关差异基因大部分上调。DGE技术为我们在分子水平上提供了PAT胚肾细胞损伤机制的众多线索,为相关基因生物学效应研究奠定基础。
[Abstract]:The dynamic changes of gene expression profile during the process of human embryonic kidney cell injury induced by food contaminant Rod toxin were studied. The differentially expressed genes were screened by digital gene expression profiling (DGE) technique in HEK293 cells treated with 5 渭 M PAT for 3h. The gene enriched KEGG signaling pathway and Pathway signaling pathway were analyzed, and some differentially expressed genes were verified by quantitative PCR. The results showed that there were 79 and 261 differentially expressed genes induced by PAT at 3 h and 24 h respectively, among which 71 genes were up-regulated and 192 genes were up-regulated. The down-regulated genes were 8 and 69. Pat mainly induced the differential expression of apoptotic genes. All the differentially expressed genes upregulated and down-regulated the differentially expressed genes of RNA synthesis. Ubiquitin mediated protein degradation mostly upregulated MAPK signaling pathway mainly involved in downstream process of PAT toxicity. Most of its differential genes up-regulated. DGE technology provides us with many clues on the mechanism of PAT embryonic kidney cell injury at the molecular level and lays a foundation for the study of the biological effects of related genes.
【作者单位】：西北农林科技大学食品科学与工程学院;兰州理工大学生命科学与工程学院;
【基金】：国家自然科学基金资助项目(31571928、31201286) 中央高校基本科研创新一般项目(2014YB021)
【分类号】：R99

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