聚乙二醇琥珀酸酯1000与Soluplus对人参皂苷CK在Caco-2细胞模型上转运的影响

发布时间：2018-05-19 21:04

  本文选题：人参皂苷CK + Caco-细胞模型　； 参考：《中国药学杂志》2015年22期

【摘要】：目的考察聚乙二醇琥珀酸酯1000(TPGS 1000)与Soluplus对人参皂苷CK在Caco-2细胞模型上转运的影响。方法采用Caco-2细胞模型研究不同浓度的聚乙二醇琥珀酸酯1000与Soluplus对人参皂苷CK细胞转运行为的影响,以超高压液相色谱法(UPLC)测定细胞样品溶液中人参皂苷CK的浓度,计算表观渗透系数(Papp)与外排比率。结果当人参皂苷CK与聚乙二醇琥珀酸酯1000或Soluplus质量比例分别为1∶1、1∶3和1∶9时,人参皂苷CK吸收显著增加,外排显著降低,外排比率也随之显著下降(P0.05);在相同比例条件下,聚乙二醇琥珀酸酯1000对人参皂苷CK的促吸收效果和抑制外排效果均优于Soluplus(P0.05)。结论在Caco-2细胞模型上,聚乙二醇琥珀酸酯1000与Soluplus均可显著促进人参皂苷CK的吸收,有希望应用于CK制剂的开发中。
[Abstract]:Aim to investigate the effects of polyethylene glycol succinate (1000(TPGS 1000) and Soluplus on the transport of ginsenoside CK on Caco-2 cell model. Methods Caco-2 cell model was used to study the effects of different concentrations of polyethylene glycol succinate 1000 and Soluplus on the transport behavior of ginsenoside CK cells. The concentration of ginsenoside CK in cell sample solution was determined by ultrahigh pressure liquid chromatography (HPLC). The apparent permeability coefficient (Pappa) and efflux ratio were calculated. Results when the mass ratio of ginsenoside CK to polyethylene glycol succinate 1000 or Soluplus was 1: 1: 1: 3 and 1:9, the absorption of ginsenoside CK was significantly increased, and the efflux of ginsenoside CK was significantly decreased. The efflux ratio also decreased significantly (P0.05A), and at the same ratio, the effects of polyethylene glycol succinate 1000 on the absorption and inhibition of ginsenoside CK were better than that of Soluplusp 0.05%. Conclusion on Caco-2 cell model, both polyethylene glycol succinate 1000 and Soluplus can significantly promote the absorption of ginsenoside CK.
【作者单位】： 江苏省中医药研究院国家中医药管理局中药释药系统重点研究室;宿迁市第一人民医院;
【基金】：国家自然科学基金资助项目(81403119)
【分类号】：R969.2
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本文编号：1911663