雷贝拉唑对非甾体抗炎药诱导小肠损伤的保护作用及可能机制研究

发布时间：2018-05-21 07:56

本文选题：雷贝拉唑 + 非甾体类消炎药　；参考：《中国现代医学杂志》2015年20期

【摘要】：目的探讨质子泵抑制剂(PPIs)药物雷贝拉唑对非甾体抗炎药(NISADs)吲哚美辛诱导小肠损伤的保护作用及可能机制。方法检测PPIs雷贝拉唑对吲哚美辛诱导小肠损伤的保护作用及与HO-1的相关性。给予小鼠口服吲哚美辛10 mg/kg,24 h后处死。在给予吲哚美辛前30 min,口服雷贝拉唑30~100 mg/kg和奥美拉唑30~100 mg/kg;在给予吲哚美辛或雷贝拉唑前10 min,肌内注射HO-1抑制剂锡-原卟啉(Sn PP)30 mg/kg。结果吲哚美辛引起小肠出血性损伤,侵袭性肠道杆菌数量增加,诱导型一氧化氮合酶(i NOS)表达和髓过氧化物酶(MPO)活性增加。雷贝拉唑以剂量依赖性保护吲哚美辛引起的肠损伤,并且MPO活性上升受到抑制,而奥美拉唑无该作用。预处理Sn PP能够加重吲哚美辛引起的小肠损伤,并拮抗雷贝拉唑的保护作用。结论雷贝拉唑能够保护吲哚美辛诱导的小肠损伤,并与雷贝拉唑通过上调黏膜中HO-1/CO的表达而抑制i NOS表达。
[Abstract]:Objective to investigate the protective effect of Rapeprazole, a proton pump inhibitor (PPIsa), on intestinal injury induced by non-steroidal anti-inflammatory drug NISADsIndomethacin. Methods the protective effect of PPIs rabeprazole on indomethacin induced intestinal injury and its correlation with HO-1 were detected. Mice were killed 24 hours after oral administration of indomethacin 10 mg / kg. 30 minutes before the administration of indomethacin, Rabeprazole 30 mg/kg and omeprazole 30 mg / kg were given orally, and HO-1 inhibitor Sn PP)30 mg / kg was injected intramuscularly 10 minutes before the administration of indomethacin or rabeprazole. Results Indomethacin induced intestinal hemorrhagic injury, increased the number of invasive enterobacterias, increased the expression of inducible nitric oxide synthase (iNOS) and the activity of myeloperoxidase (MPO). Rabeprazole protects intestinal injury induced by indomethacin in a dose-dependent manner, and the increase of MPO activity is inhibited, but omeprazole does not. Sn-PP pretreatment could aggravate the intestinal injury induced by indomethacin and antagonize the protective effect of rabeprazole. Conclusion Rabeprazole can protect the small intestine injury induced by indomethacin, and both Rabeprazole and Rabeprazole inhibit the expression of I NOS by upregulating the expression of HO-1/CO in mucous membrane.
【作者单位】：中山大学孙逸仙纪念医院;
【分类号】：R96

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