基于生物质谱的蛋白质定量技术及其在药代动力学研究中的应用

发布时间：2018-05-21 09:56

本文选题：生物质谱 + 蛋白质定量技术　；参考：《中国药科大学学报》2015年02期

【摘要】：蛋白质定量技术是指对细胞、组织以至整体生物体内的蛋白质表达水平进行全谱定量分析的手段,其在生物过程机制的探索、生物标志物和药物作用靶标的发现及验证等过程中发挥着重要作用。新兴生物质谱技术和数据采集方法的发展为蛋白质定量提供了先进的技术平台,促使了蛋白质研究逐渐从简单定性转变为精确定量。本文综述了基于生物质谱的蛋白分子定量策略及方法,评述了不同方法的特点和适用范围,并阐述了其在药物代谢动力学研究领域,如药物转运体、药物代谢酶的定量和多肽及蛋白类药物的药代动力学研究等方面的应用,为传统的药代动力学研究提供了新的思路与方法。
[Abstract]:Protein quantitative technique refers to the means of full spectrum quantitative analysis of protein expression level in cells, tissues and even the whole organism, and the exploration of its mechanism in biological process. The discovery and verification of biomarkers and drug targets play an important role. The development of new biological mass spectrometry and data acquisition methods provides an advanced technology platform for protein quantification, which promotes protein research to change from simple qualitative analysis to accurate quantification. In this paper, the strategies and methods of protein molecular quantification based on bio-mass spectrometry are reviewed. The characteristics and application of different methods are reviewed, and their applications in the field of pharmacokinetics, such as drug transporters, are reviewed. The application of quantification of drug metabolizing enzymes and pharmacokinetics of peptides and proteins provides a new way of thinking and method for traditional pharmacokinetic research.
【作者单位】：中国药科大学药物代谢动力学重点实验室;
【基金】：国家自然科学基金资助项目(No.81403005,No.81273589) 江苏省自然科学基金资助项目(No.BK20140667)~~
【分类号】：R96

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