氯沙坦对大鼠废用性骨骼肌不同肌球蛋白亚型基因表达的影响

发布时间：2018-05-22 17:57

  本文选题：骨骼肌 + 萎缩　； 参考：《西安体育学院学报》2017年04期

【摘要】：目的探讨氯沙坦对大鼠废用性骨骼肌不同肌球蛋白亚型基因表达的影响。方法采用大鼠尾部悬吊2周建立废用性肌肉萎缩模型,药物干预组给予氯沙坦灌胃2周。采用实时荧光定量PCR测试比目鱼肌中不同肌球蛋白亚型基因表达量,冰冻切片测试骨骼肌中毛细血管密度和毛细血管数/肌纤维数比值。结果 (1)与C组相比,TS组大鼠体重、比目鱼肌重量和相对重量均降低(P0.05或P0.01),与TS组相比,TD组大鼠体重、比目鱼肌重量和相对重量均升高(P0.05);(2)与C组相比,TS组、TD组大鼠比目鱼肌毛细血管密度和毛细血管数/肌纤维数比值显著下降(P0.05),TD组与TS组相比有显著性增加(P0.05);(3)与C组相比,TS、TD组的MHC I mRNA、MHCⅡa mRNA、MHCⅡb mRNA表达量均降低(P0.01、P0.05、P0.05),TD组的MHC I mRNA表达量与TS组相比升高(P0.05)。结论大鼠尾部悬吊14天,比目鱼肌出现明显萎缩。服用氯沙坦可通过改善比目鱼肌的血液循环,提高MHC I mRNA的表达,具有一定的抑制骨骼肌萎缩作用。
[Abstract]:Objective to investigate the effect of losartan on gene expression of different myosin subtypes in rat skeletal muscle. Methods the rat model of disused muscular atrophy with tail suspension for 2 Zhou Jianli was used. Losartan was administered intragastrically for 2 weeks in the drug intervention group. The gene expression of different myosin subtypes in soleus muscle was measured by real-time fluorescence quantitative PCR, and the capillary density and the ratio of capillary number to muscle fiber number in skeletal muscle were measured by frozen sections. Results compared with group C, the body weight, soleus muscle weight and relative weight of TS group were decreased by P0.05 or P0.01, and those of TD group were significantly lower than those of TS group. Compared with group C, the density of capillaries and the ratio of capillaries to fiber number of soleus muscle in TD group decreased significantly. The ratio of capillary number to muscle fiber number in group TD was significantly higher than that in group T (P 0.05) and group C was significantly higher than that in group TS. The expression of MHC I mRNA-MHC 鈪，

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