pDNA-巯基壳聚糖纳米粒的制备及Box-Behnken效应面法工艺优化

发布时间：2018-05-25 22:02

本文选题：巯基壳聚糖 + 质粒DNA　；参考：《华侨大学学报(自然科学版)》2017年05期

【摘要】：以巯基壳聚糖(TCS)为基因载体,采用离子交联法制备能用于基因口服研究的质粒DNA-巯基壳聚糖纳米粒(pDNA-TCS-NPs).分别以TCS质量浓度、三聚磷酸钠(TPP)质量浓度、pH值和转速为考察对象,以pDNA-TCS-NPs粒径和Zeta电位为评价指标,采用4因素3水平Box-Behnken效应面法筛选最佳制备工艺,并对其外观形态,包封率等体外性质进行考察.结果表明:TCS质量浓度为0.80mg·mL~(-1),TPP质量浓度为0.65mg·mL~(-1),pH=5.3,转速为2 000r·min~(-1)是最优制备工艺,可制得粒径为(134.21±1.34)nm,Zeta电位为(24.36±0.29)mV,包封率在(80.26±0.56)%,形状规则且分散良好的pDNA-巯基壳聚糖纳米粒;Box-Behnken实验设计可用于预测和优化pDNA-TCS-NP制备工艺优化筛选.
[Abstract]:Plasmid DNA-mercaptochitosan nanoparticles pDNA-TCS-NPsN were prepared by ion crosslinking method with thioglycol-chitosan (TCSs) as gene carrier. The pH value and rotational speed of TCS concentration and pDNA-TCS-NPs concentration were investigated respectively. The pDNA-TCS-NPs particle size and Zeta potential were used as evaluation indexes. The best preparation process was selected by 4 factors and 3 levels Box-Behnken effect surface method, and the appearance of pDNA-TCS-NPs was analyzed. The entrapment efficiency was investigated in vitro. The results showed that the optimal preparation technology was 0.80mg mLL ~ (-1), pH = 5.3 and rotational speed of 2 000 r 路min ~ (-1), respectively, and the results showed that the optimum preparation conditions were as follows: (1) the mass concentration of TCS was 0.80mg mLX ~ (-1) and the mass concentration of TCS was (2 000 r 路min ~ -1). The Zeta potential is 24.36 卤0.29mV, the entrapment efficiency is 80.26 卤0.56mV.The Box-Behnken nanoparticles with regular shape and good dispersion can be used to predict and optimize the preparation process of pDNA-TCS-NP.
【作者单位】：华侨大学生物医学学院;兰州军区总医院临床药学科;厦门大学药学院;
【基金】：国家自然科学基金资助项目(81302652) 福建省自然科学基金资助项目(2015J01342)
【分类号】：R945

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