内毒素耐受对细胞因子的影响

发布时间：2018-05-25 23:25

本文选题：内毒素耐受 + 肿瘤坏死因子α　；参考：《中国医科大学学报》2015年03期

【摘要】：目的进一步明确内毒素耐受时炎性相关细胞因子的变化。方法昆明鼠分组处置。建立内毒素(LPS)耐受鼠模型。ELISA方法测定血清肿瘤坏死因子α(TNF-α),白细胞介素10(IL-10)和白细胞介素17(IL-17)浓度。结果 LPS对照组血清TNF-α、IL-10和IL-17浓度分别为(2 261.49±1 090.28)pg/m L,(7 780.42±2 320.04)pg/m L,(27.28±13.76)pg/m L,较健康对照组显著增高。而内毒素耐受组相应细胞因子血清浓度分别为(1 174.19±450.91)pg/m L,(1 293.26±520.85)pg/m L,(22.26±11.44)pg/m L。地塞米松干预进一步降低了TNF-α和IL-17的水平,但对IL-10水平无显著影响。结论内毒素耐受状态与内毒素炎症状态比较,TNF-α和IL-10水平显著降低,IL-17水平无显著变化。应用地塞米松可进一步降低内毒素耐受状态下的TNF-α和IL-17水平。
[Abstract]:Objective to investigate the changes of inflammatory cytokines during endotoxin tolerance. Methods Kunming rats were divided into groups. A rat model of endotoxin tolerance was established. The serum levels of TNF- 伪, IL-10 and IL-17 were determined by Elisa. Results the serum levels of TNF- 伪 IL-10 and IL-17 in LPS group were 2 261.49 卤1 090.28)pg/m / L, 7 780.42 卤2 320.04)pg/m / L and 27.28 卤13.76)pg/m / L, respectively, which were significantly higher than those in healthy control group. In endotoxin tolerance group, the serum concentration of cytokines was 1 174.19 卤450.91)pg/m / L, 1 293.26 卤520.85)pg/m / L, 22.26 卤11.44)pg/m / L, respectively. Dexamethasone further reduced the levels of TNF- 伪 and IL-17, but had no significant effect on IL-10 levels. Conclusion the levels of TNF- 伪 and IL-10 in endotoxin tolerance state and endotoxin inflammation state were significantly lower than that in endotoxin inflammatory state. Dexamethasone could further reduce the levels of TNF- 伪 and IL-17 in endotoxin tolerant state.
【作者单位】：中国医科大学附属第四医院风湿免疫科;
【分类号】：R965

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