高效液相色谱串联质谱法研究环磷酰胺线性微透析探针体内外校正影响因素

发布时间：2018-05-30 17:45

本文选题：微透析 + 环磷酰胺　；参考：《国际药学研究杂志》2015年03期

【摘要】：目的建立环磷酰胺线性探针体内外回收率最优校正方法。方法以复方氯化钠注射液为灌注液,反向透析校正方法中的增量法与减量法校正线性探针,考察不同灌注流速对环磷酰胺线性探针回收率的影响。结果灌注流速为1.0μl/min时,环磷酰胺回收率大幅提高,增量法与减量法回收率分别由(24.11±1.75)%、(26.19±0.76)%提升至(50.93±0.91)%、(53.31±1.23)%,体内回收率为(26.19±0.76)%。结论环磷酰胺适宜进行微透析技术的取样,本实验校正方法简便有效。
[Abstract]:Objective to establish the optimal correction method for the recovery rate of cyclophosphamide linear probe in vivo and in vitro. Method using Compound Sodium Chloride Injection as the perfusion liquid, the increment method and the reduction method in the reverse dialysis correction method to correct linear probe, and investigate the effect of different perfusion flow velocity on the recovery rate of cyclophosphamide linear probe. The recovery rate of phosphamide was greatly improved, the recovery rate of the incremental method and the reduction method were (24.11 + 1.75)%, (26.19 + 0.76)%, (50.93 + 0.91)%, (53.31 + 1.23)% and (26.19 + 0.76)%, and the recovery rate in the body was (26.19 + 0.76)%. Conclusion cyclophosphamide is suitable for microdialysis sampling. The correction method of this experiment is simple and effective.
【作者单位】：第二炮兵总医院;解放军153医院;解放军第101医院;
【基金】：国家自然科学基金青年项目资助(81301662)
【分类号】：R927.11;O657.63

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