排卵后给予米非司酮对雌性大鼠动情周期、黄体功能的影响及意义

发布时间：2018-05-31 17:51

本文选题：黄体功能不全 + 米非司酮　；参考：《山东医药》2017年44期

【摘要】：目的探讨排卵后给予米非司酮对雌性大鼠动情周期、黄体功能的影响及其意义。方法选择具有规律周期性变化、动情周期为4天的雌性大鼠40只,随机分为空白组、单周期组、两周期组及三周期组,每组10只。单周期组、两周期组及三周期组分别于排卵后灌胃给予米非司酮1 m L/100 g,分别用药1、2、3个周期;空白组灌胃给予等体积生理盐水。记录各组给药前后动情后期及动情周期的持续时间,统计出现动情周期紊乱的大鼠数量。末次给药后第2天(给药后D2)及给药后进入下一动情周期的第2天(下一周期D2)采用放射免疫分析法检测血清雌二醇(E2)、孕酮(P)、促卵泡生成素(FSH)、促黄体生成素(LH)水平,处死后计数卵巢组织中生长卵泡、黄体及闭锁卵泡数,采用实时荧光定量PCR法检测子宫组织雌激素受体(ER)、孕激素受体(PR)mRNA表达。结果空白组、单周期组动情周期均保持规律性,两周期组、三周期组中分别有1只(10%)、5只(50%)出现动情周期紊乱。各组给药前后动情周期持续时间无明显变化(P均0.05);两周期组、三周期组动情后期持续时间均明显短于给药前及空白组、单周期组给药后(P均0.05)。单周期组、两周期组、三周期组给药后D2、下一周期D2血清E2均高于空白组,且三周期组升高更明显(P均0.05)。单周期组、两周期组、三周期组给药后D2血清FSH均低于空白组,LH均高于空白组(P均0.05)。两周期组、三周期组下一周期D2血清FSH均低于空白组与单周期组,血清LH均高于空白组与单周期组(P均0.05)。各组给药后D2、下一周期D2血清P水平比较差异均无统计学意义(P均0.05)。单周期组、两周期组及三周期组闭锁卵泡数均多于空白组,黄体数、生长卵泡数均少于空白组(P均0.05)。两周期组与三周期组生长卵泡数、黄体数及闭锁卵泡数比较均无统计学差异(P均0.05)。单周期组、两周期组、三周期组子宫组织ER mRNA相对表达量均高于空白组,且两周期组、三周期组升高更明显(P均0.05)。单周期组、两周期组、三周期组子宫组织PR mRNA相对表达量均高于空白组,且三周期组升高更明显(P均0.05)。结论雌性大鼠排卵后给予两个周期米非司酮可通过提高子宫组织PR基因表达而缩短其动情后期持续时间并导致黄体功能不全,可用于建立有效、稳定的黄体功能不全大鼠模型。
[Abstract]:Objective to investigate the effect of mifepristone on estrous cycle and luteal function in female rats after ovulation. Methods Forty female rats with a regular and periodic estrous cycle of 4 days were randomly divided into three groups: blank group, single cycle group, two cycle group and three cycle group with 10 rats in each group. One cycle group, two cycle group and three cycle group were given mifepristone 1 mL / 100 g intragastrically after ovulation. The duration of estrus and estrus cycle before and after administration were recorded and the number of rats with estrous cycle disorder was counted. The serum levels of estradiol E _ (2), progesterone, follicle stimulating hormone (FSH) and luteinizing hormone (LH) were measured by radioimmunoassay on the second day after the last administration (D2) and the second day after the next estrus cycle (D2). The number of follicle growth, corpus luteum and atresia follicles in ovarian tissues were counted after death. The expression of estrogen receptor ERN and progesterone receptor PRN mRNA in uterine tissue was detected by real-time fluorescence quantitative PCR. Results the estrus cycle was regular in blank group and single cycle group. In two cycle group and three cycle group, there was a disturbance of estrous cycle in 10 cases and 5 cases in three cycle group respectively. The duration of estrous cycle before and after administration was not significantly changed in each group (P < 0.05), the duration of estrus in the group of two cycles and the group of three cycles was significantly shorter than that of the group before and after administration of the drug, and the duration of estrus in the group of single cycle was 0.05g after the administration of the drug. The levels of serum E _ 2 in D _ 2, D _ 2 and D _ 2 in the next cycle were higher than those in the control group, and the levels of serum E _ 2 in the three cycle group were significantly higher than those in the control group (P < 0.05). The levels of serum FSH in single cycle group, two cycle group and three cycle group were lower than those in blank group (P < 0.05). In the two cycle group, the next cycle of three cycle group, the serum FSH of D2 was lower than that of the blank group and the single cycle group, and the serum LH was higher than that of the blank group and the single cycle group (P 0.05). There was no significant difference in D2P levels in D2 and D2P in the next cycle after administration in each group (P = 0.05). The number of atresia follicles in single cycle group, two cycle group and three cycle group were more than that in blank group, the number of corpus luteum and the number of growing follicles were lower than that of blank group (P < 0.05). There was no significant difference in the number of growth follicles, luteal bodies and atresia follicles between two and three cycle groups (P < 0.05). The relative expression of ER mRNA in uterine tissues of single cycle group, two cycle group and three cycle group was higher than that of blank group, and the increase of ER mRNA in two cycle group was more obvious than that in three cycle group (P 0.05). The relative expression of PR mRNA in single cycle group, two cycle group and three cycle group was higher than that in blank group, and the increase of PR mRNA in three cycle group was more obvious than that in control group (P < 0.05). Conclusion two cycles of mifepristone after ovulation in female rats can shorten the duration of late estrus and lead to luteal insufficiency by increasing the expression of PR gene in uterine tissue. It can be used to establish an effective and stable rat model of luteal insufficiency.
【作者单位】：南京中医药大学;
【基金】：国家自然科学基金资助项目(81473713) 江苏省自然科学基金资助项目(BK20141463) 江苏省普通高校研究生科研创新计划项目(KYLX15_0991) 中国和欧洲医疗保健发展合作研究计划(CHETCH)
【分类号】：R965

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