来曲唑和脱氢表雄酮建立肾虚痰湿特征信息肥胖PCOS大鼠动物模型的对比研究

发布时间：2018-06-01 12:17

本文选题：肥胖多囊卵巢综合征 + 来曲唑　；参考：《中国中医基础医学杂志》2015年05期

【摘要】：目的:对比来曲唑和脱氢表雄酮(Polycystic ovarian syndrome,DHEA)分别加高脂乳剂制作SD雌性大鼠肾虚痰湿特征信息肥胖PCOS动物模型的优缺点。方法:观察大鼠动情周期、体质量增加幅度,测定血清性激素、血脂、相关脏器指数水平和卵巢HE染色。结果:来曲唑联合高脂模型组大鼠体质量增加幅度、FSH、LH均极显著高于空白对照组和高脂模型组,LDL、TC、TG均显著高于余3组;DHEA联合高脂模型组大鼠E2、T均极显著高于空白对照组和高脂模型组,TC、TG、LDL均极显著高于其余3组;来曲唑模型组、来曲唑联合高脂模型组、DHEA模型组、DHEA联合高脂模型组大鼠卵巢HE染色均呈现多囊样改变。结论:来曲唑加高脂乳剂诱导是研究符合肾虚痰湿特征信息肥胖PCOS发病机制较理想的动物模型,DHEA加高脂乳剂诱导适用于血脂异常、雄激素和雌激素升高的不肥胖PCOS模型。
[Abstract]:Aim: to compare the advantages and disadvantages of letrozole and dehydroepiandrosterone polycystic ovarian syndromes (DHEAA) in the establishment of PCOS animal model of kidney deficiency and phlegm dampness information in female SD rats. Methods: the estrous cycle and the increase of body mass were observed, serum sex hormone, blood lipid, relative organ index and ovary HE staining were determined. Results: the increase of body mass of rats in letrozole combined with hyperlipidemia model group was significantly higher than that in blank control group and hyperlipidemia model group. The TG of LDL- TCN in the model group was significantly higher than that in the other 3 groups of DHEA combined with hyperlipidemia model group, which was significantly higher than that in the blank control group. The level of TGG LDL in both group and hyperlipidemia group was significantly higher than that in the other three groups. In letrozole model group, letrozole combined with hyperlipidemia model group, DHEA model group and hyperlipidemia model group showed polycystic changes in ovarian HE staining. Conclusion: letrozole combined with high fat emulsion induction is an ideal animal model to study the mechanism of obesity PCOS which accords with the characteristics of kidney deficiency and phlegm dampness. It is suitable for the PCOS model with abnormal blood lipids androgen and estrogen.
【作者单位】：成都中医药大学;成都中医药大学附属医院;
【基金】：成都中医药大学科技发展基金(ZRMS201307)-PCOS动物模型中肾虚痰湿型诊断要素的信息挖掘研究
【分类号】：R965

【参考文献】