咪唑离子液体为背景HPCE测定血清中头孢药物

发布时间：2018-06-01 22:43

本文选题：HPCE + 电解质　；参考：《化学试剂》2017年06期

【摘要】：建立了以[Bmim][BF4]离子液体为背景电解质,高效毛细管电泳同时测定血清中头孢噻肟(CTX)、头孢唑啉(CFZ)、头孢曲松(CRO)和头孢米诺(CMN)这4种头孢类药物的方法。将头孢拉定(RAD)作为内标,考察了背景电解质浓度、分离电压、溶液p H、进样时间对分离度的影响。结果发现,背景电解质浓度为30 mmol/L、分离电压为15 kV、进样时间为10 s、检测波长为250 nm时,CTX、CFZ、CRO和CMN在9 min内能得到有效地分离,在0.5~100.0 mg/L范围内有良好的线性关系(r0.999),检出限分别为0.61、0.56、0.46和0.79 mg/L,定量限分别为2.05、1.90、1.56和2.65mg/L,精密度(RSD)3.2%。该方法用于血清中头孢药物的分析,回收率为97.16%~101.40%。离子液体经CH2Cl2萃取回收,回收率达85%以上。
[Abstract]:A method for simultaneous determination of cefotaxime, cefazolin, ceftriaxone, ceftriaxone and cefminox in serum by high performance capillary electrophoresis with [Bmim] [BF4] ionic liquid as background electrolyte was established. The effects of background electrolyte concentration, separation voltage, solution pH and injection time on the separation degree were investigated using cefradine as internal standard. The results showed that the concentration of background electrolyte was 30 mmol / L, the separation voltage was 15 kV, the injection time was 10 s, and the detection wavelength was 250nm. The separation of CTX CMN and CFZO was effective within 9 min. There was a good linear relationship within the range of 0.5 ~ 100.0 mg/L, the detection limits were 0.61g / L and 0.79 mg / L, the quantification limits were 2.05 ~ 1.901.56 and 2.65 mg / L, respectively, and the precision was 3.2mg-1 路L ~ (-1). The method has been applied to the analysis of ceftillae in serum with a recovery of 97.16% and 101.40%. The recovery rate of ionic liquid was more than 85% by CH2Cl2 extraction.
【作者单位】：邢台学院化学工程与生物技术学院;江苏力维检测科技有限公司;
【基金】：邢台市科技局科技支撑项目(2014ZC104) 河北省教育厅高等学校科学研究重点项目(ZD2014210) 邢台市科技局青年科技人才资助计划项目(2012ZZ060-1) 无锡市科技发展(社会发展示范)项目(CSE12N1605)
【分类号】：O658.9;R96

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