双氢青蒿素对体外培养兔角膜上皮细胞毒性作用的实验研究

发布时间：2018-06-06 23:25

本文选题：双氢青蒿素 + 角膜上皮细胞　；参考：《眼科》2017年03期

【摘要】：目的评价双氢青蒿素对体外培养的兔角膜上皮细胞的毒性作用。设计实验研究。研究对象体外培养兔角膜上皮细胞。方法体外培养兔角膜上皮细胞,加入不同稀释浓度(1.6%、0.8%、0.4%、0.2%、0.1%、0.05%和0.025%)的双氢青蒿素共同孵育,同时以洗必泰和聚六亚甲基双胍(PHMB)做对照药物。在培养后2、6、24小时,光学显微镜下观察细胞形态,LIVE/DEAD染色观察药物作用后兔角膜上皮细胞的活性改变。主要指标细胞形态改变。结果培养后2、6小时,0.4%双氢青蒿素作用后的细胞出现圆化,细胞间隙加宽;0.0125%洗必泰作用后的细胞开始出现细胞间隙加宽,边界不清;0.003%PHMB作用后的细胞开始出现细胞边界不清,细胞内颗粒样改变。培养后24小时,0.1%的双氢青蒿素作用的角膜上皮细胞尚存在活性,0.025%的双氢青蒿素对角膜上皮细胞无明显影响。0.006%洗必泰和0.003%PHMB可导致角膜上皮细胞的死亡。结论双氢青蒿素对体外培养的兔角膜上皮细胞的毒性较低,其对活体角膜细胞的作用尚待进一步研究。
[Abstract]:Objective to evaluate the toxicity of dihydroartemisinin on rabbit corneal epithelial cells in vitro. Design experimental research. Objective to culture rabbit corneal epithelial cells in vitro. Methods Rabbit corneal epithelial cells were cultured in vitro and incubated with dihydroartemisinin with different dilution concentration of 1.6 and 0.80.42 and 0.1% and 0.025% respectively, and chlorhexidine and polyhexamethylene biguanidine (PHMBB) were used as control drugs. The morphology of rabbit corneal epithelial cells was observed under optical microscope and the activity of rabbit corneal epithelial cells was observed by Live / DEAD staining. Main outcome measures changes of cell morphology. Results the cells treated with 0.4% dihydroartemisinin for 2 hours after culture showed roundness, the cells after treatment with 0.0125% chlorhexidine, the cells with 0.0125% chlorhexidine began to widen the cell space, and the cells with 0.003 PHMB had unclear boundary. Intracellular granulocyte changes. 24 hours after culture, 0.1% dihydroartemisinin had no effect on corneal epithelial cells. 0. 006% chlorhexidine and 0.003%PHMB could cause corneal epithelial cells to die. Conclusion the toxicity of dihydroartemisinin on rabbit corneal epithelial cells in vitro is low, and the effect of dihydroartemisinin on living corneal cells remains to be further studied.
【作者单位】：首都医科大学附属北京同仁医院北京同仁眼科中心北京市眼科研究所眼科学与视觉科学北京市重点实验室;
【基金】：北京中医药科技发展面上课题专项经费(JJ2013-24)
【分类号】：R965

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